Lentibacillus halodurans sp. nov., a moderately halophilic bacterium isolated from a salt lake in Xin-Jiang, China

1 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China 2 Graduate School of the Chinese Academy of Sciences, Beijing 100049, China Correspondence Yanfen Xue xueyf{at}im.ac.cn A Gram-positive, rod-shaped, spore-forming and moderately halophilic bacterium (strain 8-1 T ) was isolated from a sediment sample of a neutral salt lake in Xin-Jiang, China. The strain grew optimally at 30 °C, pH 7.0–7.5 and 8–12 % (w/v) NaCl. The major cellular fatty acids were anteiso-C 15 : 0 , iso-C 15 : 0 and anteiso-C 17 : 0 . The predominant isoprenoid quinone was MK-7 and the cell-wall peptidoglycan contained meso -diaminopimelic acid. Phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids were found to be the major polar lipid components. The genomic DNA G+C content was 43.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain 8-1 T was closely related to Lentibacillus salarius KCTC 3911 T (98.0 % sequence similarity) and other recognized species within the genus Lentibacillus (94.5–95.9 %). The level of DNA–DNA relatedness between strain 8-1 T and L. salarius KCTC 3911 T was 40 %. Based on the phenotypic and genotypic data presented, strain 8-1 T is considered to represent a novel species of the genus Lentibacillus , for which the name Lentibacillus halodurans sp. nov. is proposed. The type strain is 8-1 T (=CGMCC 1.3702 T =DSM 18342 T ). Abbreviations: MHB, moderately halophilic bacteria The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 8-1 T is AY321433 .