Specific role of mitochondrial electron transport in blood-stage Plasmodium falciparum

One trick pony The mitochondrial electron transport chain of the malaria parasite Plasmodium falciparum serves only one function, it seems. Its sole remaining purpose is to make ubiquinone, required as an electron acceptor for a pyrimidine biosynthesis enzyme. Many single-cell eukaryotes have lost their mitochondrial genomes during evolution, and P. falciparum seems to be close to that state. The parasite's electron transport system is of particular interest as a target for antimalarials such as proguanil The origin of all mitochondria can be traced to the symbiotic arrangement that resulted in the emergence of eukaryotes in a world that was exclusively populated by prokaryotes 1 , 2 , 3 . This arrangement, however, has been in continuous genetic flux: the varying degrees of gene loss and transfer from the mitochondrial genome in different eukaryotic lineages seem to signify an ongoing ‘conflict’ between the host and the symbiont. Eukaryotic parasites belonging to the phylum Apicomplexa provide an excellent example to support this view. These organisms contain the smallest mitochondrial genomes known 4 , 5 , with an organization that differs among various genera; one genus, Cryptosporidium , seems to have lost the entire mitochondrial genome 6 , 7 . Here we show that erythrocytic stages of the human malaria parasite Plasmodium falciparum seem to maintain an active mitochondrial electron transport chain to serve just one metabolic function: regeneration of ubiquinone required as the electron acceptor for dihydroorotate dehydrogenase, an essential enzyme for pyrimidine biosynthesis. Transgenic P. falciparum parasites expressing Saccharomyces cerevisiae dihydroorotate dehydrogenase, which does not require ubiquinone as an electron acceptor 8 , were completely resistant to inhibitors of mitochondrial electron transport. Maintenance of mitochondrial membrane potential, however, was essential in these parasites, as indicated by their hypersensitivity to proguanil, a drug that collapsed the membrane potential in the presence of electron transport inhibitors. Thus, acquisition of just one enzyme can render mitochondrial electron transport nonessential in erythrocytic stages of P. falciparum .