Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detection
We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a...
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| Veröffentlicht in: | Nature methods 2007-03, Vol.4 (3), p.223-225 |
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| creator | Deufel, Christopher Forth, Scott Simmons, Chad R Dejgosha, Siavash Wang, Michelle D |
| description | We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a DNA molecule. We directly measured the torque on a single DNA molecule as it underwent a phase transition from B-form to supercoiled P-form. |
| doi_str_mv | 10.1038/nmeth1013 |
| format | Article |
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We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a DNA molecule. We directly measured the torque on a single DNA molecule as it underwent a phase transition from B-form to supercoiled P-form.</description><identifier>ISSN: 1548-7091</identifier><identifier>EISSN: 1548-7105</identifier><identifier>DOI: 10.1038/nmeth1013</identifier><identifier>PMID: 17322891</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Analysis ; Bioinformatics ; Biological Microscopy ; Biological Techniques ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; brief-communication ; Deoxyribonucleic acid ; DNA ; DNA - chemistry ; DNA - ultrastructure ; DNA sequencing ; Elasticity ; Electrochemistry - instrumentation ; Equipment Design ; Equipment Failure Analysis ; Lasers ; Life Sciences ; Methods ; Micromanipulation - instrumentation ; Micromanipulation - methods ; Molecular biology ; Nanostructures - chemistry ; Nanotechnology ; Nanotechnology - instrumentation ; Nucleic Acid Conformation ; Nucleotide sequencing ; Optics ; Proteomics ; Quartz ; Quartz - chemistry ; Research methodology ; Stress, Mechanical ; Structure ; Torque ; Transducers</subject><ispartof>Nature methods, 2007-03, Vol.4 (3), p.223-225</ispartof><rights>Springer Nature America, Inc. 2007</rights><rights>COPYRIGHT 2007 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Mar 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-dd3b59c98e4dbc8e04b863be4190d01210c49cfe01389741baece4c5f6df357b3</citedby><cites>FETCH-LOGICAL-c509t-dd3b59c98e4dbc8e04b863be4190d01210c49cfe01389741baece4c5f6df357b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nmeth1013$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nmeth1013$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17322891$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deufel, Christopher</creatorcontrib><creatorcontrib>Forth, Scott</creatorcontrib><creatorcontrib>Simmons, Chad R</creatorcontrib><creatorcontrib>Dejgosha, Siavash</creatorcontrib><creatorcontrib>Wang, Michelle D</creatorcontrib><title>Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detection</title><title>Nature methods</title><addtitle>Nat Methods</addtitle><addtitle>Nat Methods</addtitle><description>We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. 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We directly measured the torque on a single DNA molecule as it underwent a phase transition from B-form to supercoiled P-form.</description><subject>Analysis</subject><subject>Bioinformatics</subject><subject>Biological Microscopy</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>brief-communication</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA - ultrastructure</subject><subject>DNA sequencing</subject><subject>Elasticity</subject><subject>Electrochemistry - instrumentation</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Lasers</subject><subject>Life Sciences</subject><subject>Methods</subject><subject>Micromanipulation - instrumentation</subject><subject>Micromanipulation - methods</subject><subject>Molecular biology</subject><subject>Nanostructures - chemistry</subject><subject>Nanotechnology</subject><subject>Nanotechnology - 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Academic</collection><jtitle>Nature methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deufel, Christopher</au><au>Forth, Scott</au><au>Simmons, Chad R</au><au>Dejgosha, Siavash</au><au>Wang, Michelle D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detection</atitle><jtitle>Nature methods</jtitle><stitle>Nat Methods</stitle><addtitle>Nat Methods</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>4</volume><issue>3</issue><spage>223</spage><epage>225</epage><pages>223-225</pages><issn>1548-7091</issn><eissn>1548-7105</eissn><abstract>We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a DNA molecule. We directly measured the torque on a single DNA molecule as it underwent a phase transition from B-form to supercoiled P-form.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>17322891</pmid><doi>10.1038/nmeth1013</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 1548-7091 |
| ispartof | Nature methods, 2007-03, Vol.4 (3), p.223-225 |
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| subjects | Analysis Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Deoxyribonucleic acid DNA DNA - chemistry DNA - ultrastructure DNA sequencing Elasticity Electrochemistry - instrumentation Equipment Design Equipment Failure Analysis Lasers Life Sciences Methods Micromanipulation - instrumentation Micromanipulation - methods Molecular biology Nanostructures - chemistry Nanotechnology Nanotechnology - instrumentation Nucleic Acid Conformation Nucleotide sequencing Optics Proteomics Quartz Quartz - chemistry Research methodology Stress, Mechanical Structure Torque Transducers |
| title | Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detection |
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