Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detection

We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a...

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Veröffentlicht in:	Nature methods 2007-03, Vol.4 (3), p.223-225
Hauptverfasser:	Deufel, Christopher, Forth, Scott, Simmons, Chad R, Dejgosha, Siavash, Wang, Michelle D
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Deoxyribonucleic acid DNA DNA - chemistry DNA - ultrastructure DNA sequencing Elasticity Electrochemistry - instrumentation Equipment Design Equipment Failure Analysis Lasers Life Sciences Methods Micromanipulation - instrumentation Micromanipulation - methods Molecular biology Nanostructures - chemistry Nanotechnology Nanotechnology - instrumentation Nucleic Acid Conformation Nucleotide sequencing Optics Proteomics Quartz Quartz - chemistry Research methodology Stress, Mechanical Structure Torque Transducers
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Beschreibung
Zusammenfassung:	We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a DNA molecule. We directly measured the torque on a single DNA molecule as it underwent a phase transition from B-form to supercoiled P-form.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth1013