Mito-DEPMPO synthesized from a novel NH2-reactive DEPMPO spin trap: a new and improved trap for the detection of superoxide

Mito-DEPMPO, a new DEPMPO analogue bearing a triphenylphosphonium group, was synthesized via a novel NH2-reactive DEPMPO. The half-life of the Mito-DEPMPO superoxide adduct was estimated to be ca. 40 min. Using Mito-DEPMPO, reactive oxygen species generated in intact mitochondria were detected and c...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2007-01 (10), p.1083-1085
Hauptverfasser:	Hardy, Micael, Chalier, Florence, Ouari, Olivier, Finet, Jean-Pierre, Rockenbauer, Antal, Kalyanaraman, Balaraman, Tordo, Paul
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cyclic N-Oxides - chemical synthesis Cyclic N-Oxides - chemistry Electron Spin Resonance Spectroscopy Hypoxanthine - metabolism Mice Mitochondria - metabolism Osteoclasts - cytology Osteoclasts - metabolism Spin Labels Spin Trapping Superoxide Dismutase - metabolism Superoxides - metabolism Xanthine Oxidase - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Mito-DEPMPO, a new DEPMPO analogue bearing a triphenylphosphonium group, was synthesized via a novel NH2-reactive DEPMPO. The half-life of the Mito-DEPMPO superoxide adduct was estimated to be ca. 40 min. Using Mito-DEPMPO, reactive oxygen species generated in intact mitochondria were detected and characterized by EPR.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/b616076j