Fourier transform infrared microspectroscopy identifies symmetric PO(2)(-) modifications as a marker of the putative stem cell region of human intestinal crypts

Fourier transform infrared microspectroscopy identifies symmetric PO(2)(-) modifications as a marker of the putative stem cell region of human intestinal crypts

Complex biomolecules absorb in the mid-infrared (lambda = 2-20 microm), giving vibrational spectra associated with structure and function. We used Fourier transform infrared (FTIR) microspectroscopy to "fingerprint" locations along the length of human small and large intestinal crypts. Par...

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Veröffentlicht in:Stem cells (Dayton, Ohio) Ohio), 2008-01, Vol.26 (1), p.108-118
Hauptverfasser: Walsh, Michael J, Fellous, Tariq G, Hammiche, Azzedine, Lin, Wey-Ran, Fullwood, Nigel J, Grude, Olaug, Bahrami, Fariba, Nicholson, James M, Cotte, Marine, Susini, Jean, Pollock, Hubert M, Brittan, Mairi, Martin-Hirsch, Pierre L, Alison, Malcolm R, Martin, Francis L
Format: Artikel
Sprache:eng
Schlagworte:
Biomarkers - analysis
Humans
Immunohistochemistry
Intestinal Mucosa - cytology
Principal Component Analysis
Spectroscopy, Fourier Transform Infrared
Stem Cells - cytology
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Zusammenfassung:Complex biomolecules absorb in the mid-infrared (lambda = 2-20 microm), giving vibrational spectra associated with structure and function. We used Fourier transform infrared (FTIR) microspectroscopy to "fingerprint" locations along the length of human small and large intestinal crypts. Paraffin-embedded slices of normal human gut were sectioned (10 microm thick) and mounted to facilitate infrared (IR) spectral analyses. IR spectra were collected using globar (15 microm x 15 microm aperture) FTIR microspectroscopy in reflection mode, synchrotron (
ISSN:1066-5099
1549-4918
DOI:10.1634/stemcells.2007-0196