Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi

The genes of IRF-1 and IRF-7 have been cloned from the mandarin fish (Siniperca chuatsi). The IRF-1 gene has 4919 nucleotides (nt) and contains 10 exons and 9 introns, with an open reading frame (ORF) of 903nt encoding 301aa. The IRF-7 gene has 6057nt and also contains 10 exons and 9 introns, with a...

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Veröffentlicht in:	Veterinary Immunology and Immunopathology 2007-03, Vol.116 (1-2), p.26-36
Hauptverfasser:	Sun, B.J., Chang, M.X., Song, Y., Yao, W.J., Nie, P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence animal organs Animals Base Sequence Blotting, Southern - veterinary Chinese perch Cloning, Molecular codons disease resistance exons fish Gene organization genomics immune system immunity immunologic factors Interferon regulatory factor Interferon Regulatory Factor-1 - biosynthesis Interferon Regulatory Factor-1 - genetics Interferon Regulatory Factor-7 - biosynthesis Interferon Regulatory Factor-7 - genetics interferon regulatory factors interferons introns IRF-1 IRF-7 Mandarin fish messenger RNA molecular genetics Molecular Sequence Data open reading frames Perciformes Perciformes - genetics Perciformes - immunology Phylogeny physiological regulation polymerase chain reaction Real-time PCR Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA - chemistry RNA - genetics Sequence Alignment sequence analysis Siniperca chuatsi tissue distribution transcription (genetics) transcription factors Transcription, Genetic
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Zusammenfassung:	The genes of IRF-1 and IRF-7 have been cloned from the mandarin fish (Siniperca chuatsi). The IRF-1 gene has 4919 nucleotides (nt) and contains 10 exons and 9 introns, with an open reading frame (ORF) of 903nt encoding 301aa. The IRF-7 gene has 6057nt and also contains 10 exons and 9 introns, with an ORF of 1308nt encoding 436aa. The IRF-1 and IRF-7 genes have only one copy each in the genome. The transcription of IRF-1 and IRF-7 in different organs was analyzed by real-time PCR, and both molecules were constitutively expressed. The IRF-1 and IRF-7 mRNAs were abundant in gill, spleen, kidney and pronephros. The temporal transcriptional changes for IRF-1, IRF-7 and Mx were investigated within 48h after poly I: C stimulation in liver, gill, spleen and pronephros. An increased transcription was detected for IRF-1 and IRF-7 12h post-stimulation, being earlier than the transcription of Mx protein; however, IRF-1 and IRF-7 transcription decreased while the Mx protein was stable at 48h post-stimulation.
ISSN:	0165-2427 1873-2534 1365-2567
DOI:	10.1016/j.vetimm.2007.01.001