Synthesis of Modified DNA by PCR with Alkyne-Bearing Purines Followed by a Click Reaction

Synthesis of Modified DNA by PCR with Alkyne-Bearing Purines Followed by a Click Reaction

Alkyne-bearing deazapurine triphosphates were prepared and successfully incorporated into DNA using the polymerase chain reaction (PCR). The obtained alkyne-labeled DNA was successfully used in a click reaction with galactose azide.

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Veröffentlicht in:Organic letters 2008-01, Vol.10 (2), p.249-251
Hauptverfasser: Gramlich, Philipp M. E, Wirges, Christian T, Gierlich, Johannes, Carell, Thomas
Format: Artikel
Sprache:eng
Schlagworte:
Azides - chemistry
DNA - chemistry
Galactose - analogs & derivatives
Galactose - chemistry
Molecular Structure
Polymerase Chain Reaction
Purine Nucleotides - chemical synthesis
Purine Nucleotides - chemistry
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Zusammenfassung:Alkyne-bearing deazapurine triphosphates were prepared and successfully incorporated into DNA using the polymerase chain reaction (PCR). The obtained alkyne-labeled DNA was successfully used in a click reaction with galactose azide.
ISSN:1523-7060
1523-7052
DOI:10.1021/ol7026015