ISOLATION OF A RANAVIRUS FROM A GECKO (UROPLATUS FIMBRIATUS)

A virus was isolated from the liver and stomach of a leaf-tailed gecko (Uroplatus fimbriatus) with granulomatous lesions on the tongue and hepatitis. The virus was identified as an iridovirus on the basis of morphology by electron microscopy, restriction endonuclease assay, and sequencing of a large...

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Veröffentlicht in:	Journal of zoo and wildlife medicine 2005-06, Vol.36 (2), p.295-300
Hauptverfasser:	Marschang, Rachel E, Braun, Sabine, Becher, Paul
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals CASE REPORTS Cell culture techniques Cell lines DNA DNA Virus Infections - diagnosis DNA Virus Infections - veterinary Fatal Outcome Female gecko Infections Iridovirus Kidney cells Liver Lizards - virology Molecular Sequence Data Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Ranavirus Ranavirus - genetics Ranavirus - isolation & purification Reptiles Uroplatus fimbriatus virus isolation Viruses
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Beschreibung
Zusammenfassung:	A virus was isolated from the liver and stomach of a leaf-tailed gecko (Uroplatus fimbriatus) with granulomatous lesions on the tongue and hepatitis. The virus was identified as an iridovirus on the basis of morphology by electron microscopy, restriction endonuclease assay, and sequencing of a large portion of the major capsid protein gene. Comparative analysis revealed that this isolate is related to frog virus 3, the type species of the genus Ranavirus.
ISSN:	1042-7260 1937-2825
DOI:	10.1638/04-008.1