Rapid Diagnosis of Human Brucellosis by Quantitative Real-Time PCR: a Case Report of Brucellar Spondylitis

Blood samples and a bone biopsy specimen from one patient diagnosed with spondylodiscitis of unknown etiology were analyzed by quantitative real-time PCR to detect Brucella melitensis. The high sensitivity and specificity of this assay allowed the diagnosis of brucellar spondylitis within 24 h, a re...

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Veröffentlicht in:	Journal of Clinical Microbiology 2008-01, Vol.46 (1), p.385-387
Hauptverfasser:	Navarro-Martínez, Amalia, Navarro, Elena, Castaño, Maria Jesús, Solera, Javier
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged, 80 and over Bacterial arthritis and osteitis Bacterial diseases Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Blood - microbiology Bone Marrow - microbiology Brucella melitensis Brucella melitensis - genetics Brucella melitensis - isolation & purification Brucellosis - diagnosis Case Reports Female Fundamental and applied biological sciences. Psychology Human bacterial diseases Humans Infectious diseases Medical sciences Microbiology Polymerase Chain Reaction - methods Spondylitis - microbiology
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Beschreibung
Zusammenfassung:	Blood samples and a bone biopsy specimen from one patient diagnosed with spondylodiscitis of unknown etiology were analyzed by quantitative real-time PCR to detect Brucella melitensis. The high sensitivity and specificity of this assay allowed the diagnosis of brucellar spondylitis within 24 h, a result that we were unable to obtain through the use of conventional methods.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.01303-07