Microparticle-enhanced cultivation of filamentous microorganisms: Increased chloroperoxidase formation by Caldariomyces fumago as an example
Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of dif...
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| Veröffentlicht in: | Biotechnology and bioengineering 2008-02, Vol.99 (3), p.491-498 |
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| container_title | Biotechnology and bioengineering |
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| creator | Kaup, Bjoern-Arne Ehrich, Kristina Pescheck, Michael Schrader, Jens |
| description | Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Particles of ~500 μm in diameter had no effect on growth morphology or productivity of CPO formation by C. fumago. In contrast particles of |
| doi_str_mv | 10.1002/bit.21713 |
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Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Particles of ~500 μm in diameter had no effect on growth morphology or productivity of CPO formation by C. fumago. In contrast particles of <=42 μm in diameter led to the dispersion of the C. fumago mycelia up to the level of single hyphae. Under these conditions the maximum specific productivity of CPO formation was enhanced about fivefold and an accumulated CPO activity in the culture supernatant of more than 1,000 U mL⁻¹ was achieved after 10-12 days of cultivation. In addition, the novel cultivation method also showed a positive effect on growth characteristics of other filamentous microorganisms proven by the stimulation of single hyphae/cell formation. Biotechnol. Bioeng. 2008;99: 491-498. © 2007 Wiley Periodicals, Inc.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.21713</identifier><identifier>PMID: 17994590</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Ascomycota - enzymology ; Ascomycota - growth & development ; Biological and medical sciences ; Bioreactors - microbiology ; Biotechnology ; Caldariomyces fumago ; Cell culture ; Cell Culture Techniques - methods ; Cell growth ; Cell Proliferation ; Cell Survival ; Chloride Peroxidase - metabolism ; chloroperoxidase ; cultivation ; filamentous microorganisms ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; Microbial engineering. 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Bioeng</addtitle><description>Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Particles of ~500 μm in diameter had no effect on growth morphology or productivity of CPO formation by C. fumago. In contrast particles of <=42 μm in diameter led to the dispersion of the C. fumago mycelia up to the level of single hyphae. Under these conditions the maximum specific productivity of CPO formation was enhanced about fivefold and an accumulated CPO activity in the culture supernatant of more than 1,000 U mL⁻¹ was achieved after 10-12 days of cultivation. 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Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. 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Bioeng</addtitle><date>2008-02-15</date><risdate>2008</risdate><volume>99</volume><issue>3</issue><spage>491</spage><epage>498</epage><pages>491-498</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Particles of ~500 μm in diameter had no effect on growth morphology or productivity of CPO formation by C. fumago. In contrast particles of <=42 μm in diameter led to the dispersion of the C. fumago mycelia up to the level of single hyphae. 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| subjects | Ascomycota - enzymology Ascomycota - growth & development Biological and medical sciences Bioreactors - microbiology Biotechnology Caldariomyces fumago Cell culture Cell Culture Techniques - methods Cell growth Cell Proliferation Cell Survival Chloride Peroxidase - metabolism chloroperoxidase cultivation filamentous microorganisms Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Microorganisms microparticles Microspheres |
| title | Microparticle-enhanced cultivation of filamentous microorganisms: Increased chloroperoxidase formation by Caldariomyces fumago as an example |
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