A preliminary study on genotypic differences in transcript abundance of drought-responsive genes in sugar beet

In this study, four sugar beet genotypes of differing responses to drought were selected from a field experiment conducted under well-watered and water-limited conditions in 2004. In addition, two candidate genes: 2-cysteine peroxiredoxin (2-cys prx) and Nucleoside Diphosphate Kinase (NDPK), thought...

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Veröffentlicht in:Pakistan journal of biological sciences 2007-10, Vol.10 (20), p.3599-3605
Hauptverfasser: Rajabi, Abazar, Ranji, Zabihallah, Griffiths, Zhoward, Ober, Eric S
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Sprache:eng
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Zusammenfassung:In this study, four sugar beet genotypes of differing responses to drought were selected from a field experiment conducted under well-watered and water-limited conditions in 2004. In addition, two candidate genes: 2-cysteine peroxiredoxin (2-cys prx) and Nucleoside Diphosphate Kinase (NDPK), thought to be associated with drought tolerance, were chosen from a previous proteomics study in sugar beet. An expression analysis of the two drought-regulated genes using semi-quantitative reverse transcription Polymerase Chain Reaction (RT-PCR) indicated that there were genotypic differences in the transcript abundance of the candidate genes with the differences in the expression level of 2-cys prx being likely associated with the drought responses of the genotypes in a two-year field study. However, the expression analysis of the genes has to be investigated at different stages of the stress period on more genotypes.
ISSN:1028-8880
DOI:10.3923/pjbs.2007.3599.3605