Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1)

Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1)

GPR40 is G protein-coupled receptor whose endogenous ligands have recently been identified as free fatty acids (FFAs), and it has been implicated to play an important role in FFA-mediated enhancement of glucose-stimulated insulin release. We have developed a monoclonal antibody against the extracell...

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Veröffentlicht in:Biochemical and biophysical research communications 2008-01, Vol.365 (1), p.22-28
Hauptverfasser: Hirasawa, Akira, Itsubo, Chisato, Sadakane, Keiko, Hara, Takafumi, Shinagawa, Shingo, Koga, Hanako, Nose, Hiroshi, Koshimizu, Taka-aki, Tsujimoto, Gozoh
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - immunology
Antibody Specificity
Cells, Cultured
Flow cytometry
Free fatty acid receptor 1
GPR40
Humans
Immunohistochemistry
Leukocytes, Mononuclear - metabolism
Mice
Molecular Sequence Data
Monoclonal antibody
Monocytes
Peptide Fragments - metabolism
Receptors, G-Protein-Coupled - analysis
Receptors, G-Protein-Coupled - immunology
THP-1 cell
Transfection
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Zusammenfassung:GPR40 is G protein-coupled receptor whose endogenous ligands have recently been identified as free fatty acids (FFAs), and it has been implicated to play an important role in FFA-mediated enhancement of glucose-stimulated insulin release. We have developed a monoclonal antibody against the extracellular domain of GPR40. Specificity of the antibody was demonstrated by immunoprecipitation and cell surface staining using GPR40-transfected cells. GPR40 immunoreactivity was highly abundant in mouse pancreatic β-cells and splenocytes, THP-1 cells, and human peripheral blood mononuclear cells. The anti-GPR40 monoclonal antibody should prove valuable for further studying the function of this nutrient sensing receptor.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.10.142