Citrate inhibition of snake venom proteases
Thirty snake venoms had a citrate content of 2.3 to 12.9%, dry basis, by an aconitase–isocitric dehydrogenase coupled enzyme assay. This is a venom concentration range of approximately 30 to 150 mM citrate assuming 25% venom solids content. Inhibition of snake venom protease activity by the addition...
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Veröffentlicht in: | Toxicon (Oxford) 1998-12, Vol.36 (12), p.1801-1806 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Thirty snake venoms had a citrate content of 2.3 to 12.9%, dry basis, by an aconitase–isocitric dehydrogenase coupled enzyme assay. This is a venom concentration range of approximately 30 to 150
mM citrate assuming 25% venom solids content. Inhibition of snake venom protease activity by the addition of exogenous citrate was obtained using azure blue hide powder and azocasein as substrates. Protease inhibitions of 7.5% for
Crotalus atrox venom to 78% for
Bothrops picadoi venom were observed with citrate. Complete inhibition of snake venom protease activity by citrate was not observed.
Bothrops asper (Pacifico) venom showed a 41% protease inhibition by citrate with azocasein as the substrate and 46% inhibition of
Bothrops asper (Alantico) venom protease with azure blue hide power as a substrate. Trypsin was not inhibited in this system. Citrate may inhibit some venom protease activity by forming a complex with the zinc of zinc-dependent enzymes. |
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ISSN: | 0041-0101 1879-3150 |
DOI: | 10.1016/S0041-0101(98)00084-1 |