Isolation of anti-Cryptococcus neoformans immunoglobulin G

Isolation of anti-Cryptococcus neoformans immunoglobulin G

Two immunization schemes were applied to rabbits with an autoctonous strain aimed at obtaining an anti-Crytococcus neoformans IgG. Whole cells treated with formalin were used. Dosages and immunization routes were different. The sera obtained were titered and that with the highest titer was selected...

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Veröffentlicht in:Revista cubana de medicina tropical 1998, Vol.50 (1), p.27-30
Hauptverfasser: Illnait Zaragozí, M T, Martínez Machín, G, Fernández Andreu, C
Format: Artikel
Sprache:spa
Schlagworte:
Animals
Antibodies, Fungal - isolation & purification
Cryptococcus neoformans - immunology
Immunoglobulin G - isolation & purification
Rabbits
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Zusammenfassung:Two immunization schemes were applied to rabbits with an autoctonous strain aimed at obtaining an anti-Crytococcus neoformans IgG. Whole cells treated with formalin were used. Dosages and immunization routes were different. The sera obtained were titered and that with the highest titer was selected (1:1 024 by the laminar agglutination method) and purified by saline precipitation and ion exchange chromatography. An IgG of high purity and biological activity was obtained.
ISSN:0375-0760