Effects of sample preparation conditions on biomolecular solid-state NMR lineshapes

Sample preparation conditions with the 46 kDa enzyme complex of 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, shikimate-3-phosphate (S3P) and glyphosate (GLP) have been examined in an attempt to reduce linewidths in solid-state NMR spectra. The linewidths of 31P resonances associated with enz...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of biomolecular NMR 1998-10, Vol.12 (3), p.417-421
Hauptverfasser:	Jakeman, D L, Mitchell, D J, Shuttleworth, W A, Evans, J N
Format:	Artikel
Sprache:	eng
Schlagworte:	3-Phosphoshikimate 1-Carboxyvinyltransferase Alkyl and Aryl Transferases - chemistry Bacterial Proteins - chemistry Freezing Glycine - analogs & derivatives Glycine - chemistry Glyphosate Macromolecular Substances Magnetic Resonance Spectroscopy - methods NMR Nuclear magnetic resonance Polyethylene Glycols Sample preparation Shikimic Acid - analogs & derivatives Shikimic Acid - chemistry Specimen Handling Spectrum analysis Trehalose
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Sample preparation conditions with the 46 kDa enzyme complex of 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, shikimate-3-phosphate (S3P) and glyphosate (GLP) have been examined in an attempt to reduce linewidths in solid-state NMR spectra. The linewidths of 31P resonances associated with enzyme bound S3P and GLP in the lyophilized ternary complex have been reduced to 150 +/- 12 Hz and 125 +/- 7 Hz respectively, by a variety of methods involving additives and freezing techniques.
ISSN:	0925-2738 1573-5001
DOI:	10.1023/A:1008305118426