Analysis of epinephrine from fifteen different dental anesthetic formulations by capillary electrophoresis

A robust method for the quantification of epinephrine from 15 different commercial dental anesthetic formulations is developed using CE. This work presents an extension to a method reported earlier. The solvability of several anesthetic compounds was improved through appropriate dilutions and the addition of sodium dodecyl sulfate to the separation background electrolyte. By controlling the mobility of the analyte at different pH values, a dilute solution of epinephrine is focused into a sharp zone with the injection of about 150 nl of anesthetic solution into the capillary. This on-column concentration technique extended the concentration detection limit of epinephrine to about 5.0×10−7M using a commercially available UV detector. A correlation plot between the measured and listed epinephrine concentration for the 15 dental anesthetic solutions demonstrated excellent accuracy of this method.