Effect of Recombinant Human Insulin-Like Growth Factor-I on Expression of Glucose Transporters, GLUT 2 and GLUT 4, in Streptozotocin-Diabetic Rat

We investigated the effect of recombinant insulin-like growth factor-I (rhIGF-I) on the expression of glucose transporters, GLUT 2 in the liver and GLUT 4 in the heart, in streptozotocin (STZ)-diabetic rats by the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. GLU...

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Veröffentlicht in:	Japanese Journal of Pharmacology 1998, Vol.78(1), pp.63-67
Hauptverfasser:	Takashi, Asada, Toshikazu, Ogawa, Michio, Iwai, Masakazu, Kobayashi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blood Glucose - drug effects Blood Glucose - metabolism Body Weight - drug effects Diabetes Mellitus, Experimental - genetics Diabetes Mellitus, Experimental - metabolism Gene Expression - drug effects Glucose transporter Glucose Transporter Type 2 Glucose Transporter Type 4 Heart - drug effects Hypoglycemic Agents - pharmacology Immunohistochemistry Insulin - pharmacology Insulin-Like Growth Factor I - pharmacology Liver - drug effects Liver - metabolism Male Monosaccharide Transport Proteins - analysis Monosaccharide Transport Proteins - genetics Muscle Proteins Myocardium - metabolism Rats Rats, Sprague-Dawley Recombinant Proteins - pharmacology Reverse Transcriptase Polymerase Chain Reaction Reverse transcription-polymerase chain reaction (RT-PCR) RNA, Messenger - drug effects RNA, Messenger - metabolism
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Zusammenfassung:	We investigated the effect of recombinant insulin-like growth factor-I (rhIGF-I) on the expression of glucose transporters, GLUT 2 in the liver and GLUT 4 in the heart, in streptozotocin (STZ)-diabetic rats by the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. GLUT 2 and its mRNA in the liver was elevated, whereas GLUT 4 and its mRNA in the heart were decreased in STZ-diabetic rats. A two-week treatment with rhIGF-I mostly restored the expression of GLUT 2 and GLUT 4 to normal rat level. We demonstrated that the effect of IGF-I on the expressions of GLUTs was almost the same as that of insulin.
ISSN:	0021-5198 1347-3506
DOI:	10.1254/jjp.78.63