Insulin-Like Growth Factor-1 Modulation of Intestinal Epithelial Cell Restitution
After superficial intestinal injury, the mucosal integrity is reestablished by rapid migration of epithelial cells from the adjacent area in a process called restitution. Our previous study suggested that growth hormone improves intestinal healing in an experimental small bowel ulceration, mediated...
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| Veröffentlicht in: | JPEN. Journal of parenteral and enteral nutrition 1999-09, Vol.23 (5_suppl), p.S89-S92 |
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| container_title | JPEN. Journal of parenteral and enteral nutrition |
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| creator | Kai Chen Nezu, Riichiro Wasa, Masafumi Sando, Kinya Kamata, Shinkichi Takagi, Yoji Okada, Akira |
| description | After superficial intestinal injury, the mucosal integrity is reestablished by rapid migration of epithelial cells from the adjacent area in a process called restitution. Our previous study suggested that growth hormone improves intestinal healing in an experimental small bowel ulceration, mediated by insulin-like growth factor-1 (IGF-1). The aim of the present study was to assess the role of IGF-1 in mucosal epithelial restitution using an in vitro epithelial wound model. Wounds were established in confluent monolayers of the intestinal cell line, IEC-6. Migration was quantitated in the presence or absence of IGF-1 as the number of cells migrating across the wound edge. Proliferation was assessed by thymidine incorporation. IGF-1-enhanced epithelial cell migration by 2- to 2.5-fold after 12- and 24-hour treatment, respectively, the first step involved in gastrointestinal wound healing. Cell proliferation was significantly stimulated by IGF-1 as well. In addition, expression of transforming growth factor-β (TGF-β) mRNA was significantly enhanced in the wounded monolayers treated with IGF-1. IGF-1 receptor mRNA was found to be detectable throughtout the gastrointestinal mucosa and in the intestinal epithelial cells. In conclusion, these findings suggest that IGF-1 plays an important role in reconstitution of intestinal epithelial integrity after mucosal injury. (Journal of Parenteral and Enteral Nutrition23: S89-S92, 1999) |
| doi_str_mv | 10.1177/014860719902300522 |
| format | Article |
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Our previous study suggested that growth hormone improves intestinal healing in an experimental small bowel ulceration, mediated by insulin-like growth factor-1 (IGF-1). The aim of the present study was to assess the role of IGF-1 in mucosal epithelial restitution using an in vitro epithelial wound model. Wounds were established in confluent monolayers of the intestinal cell line, IEC-6. Migration was quantitated in the presence or absence of IGF-1 as the number of cells migrating across the wound edge. Proliferation was assessed by thymidine incorporation. IGF-1-enhanced epithelial cell migration by 2- to 2.5-fold after 12- and 24-hour treatment, respectively, the first step involved in gastrointestinal wound healing. Cell proliferation was significantly stimulated by IGF-1 as well. In addition, expression of transforming growth factor-β (TGF-β) mRNA was significantly enhanced in the wounded monolayers treated with IGF-1. IGF-1 receptor mRNA was found to be detectable throughtout the gastrointestinal mucosa and in the intestinal epithelial cells. In conclusion, these findings suggest that IGF-1 plays an important role in reconstitution of intestinal epithelial integrity after mucosal injury. (Journal of Parenteral and Enteral Nutrition23: S89-S92, 1999)</description><identifier>ISSN: 0148-6071</identifier><identifier>EISSN: 1941-2444</identifier><identifier>DOI: 10.1177/014860719902300522</identifier><identifier>PMID: 10483904</identifier><identifier>CODEN: JPENDU</identifier><language>eng</language><publisher>Thousand Oaks, CA: SAGE Publications</publisher><subject>Animals ; Cell Division - drug effects ; Cell Line ; Humans ; Insulin-Like Growth Factor I - physiology ; Intestinal Mucosa - drug effects ; Intestines - injuries ; RNA, Messenger - isolation & purification ; Wound Healing - drug effects</subject><ispartof>JPEN. 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IGF-1-enhanced epithelial cell migration by 2- to 2.5-fold after 12- and 24-hour treatment, respectively, the first step involved in gastrointestinal wound healing. Cell proliferation was significantly stimulated by IGF-1 as well. In addition, expression of transforming growth factor-β (TGF-β) mRNA was significantly enhanced in the wounded monolayers treated with IGF-1. IGF-1 receptor mRNA was found to be detectable throughtout the gastrointestinal mucosa and in the intestinal epithelial cells. In conclusion, these findings suggest that IGF-1 plays an important role in reconstitution of intestinal epithelial integrity after mucosal injury. 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Journal of parenteral and enteral nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kai Chen</au><au>Nezu, Riichiro</au><au>Wasa, Masafumi</au><au>Sando, Kinya</au><au>Kamata, Shinkichi</au><au>Takagi, Yoji</au><au>Okada, Akira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insulin-Like Growth Factor-1 Modulation of Intestinal Epithelial Cell Restitution</atitle><jtitle>JPEN. Journal of parenteral and enteral nutrition</jtitle><addtitle>JPEN J Parenter Enteral Nutr</addtitle><date>1999-09</date><risdate>1999</risdate><volume>23</volume><issue>5_suppl</issue><spage>S89</spage><epage>S92</epage><pages>S89-S92</pages><issn>0148-6071</issn><eissn>1941-2444</eissn><coden>JPENDU</coden><abstract>After superficial intestinal injury, the mucosal integrity is reestablished by rapid migration of epithelial cells from the adjacent area in a process called restitution. Our previous study suggested that growth hormone improves intestinal healing in an experimental small bowel ulceration, mediated by insulin-like growth factor-1 (IGF-1). The aim of the present study was to assess the role of IGF-1 in mucosal epithelial restitution using an in vitro epithelial wound model. Wounds were established in confluent monolayers of the intestinal cell line, IEC-6. Migration was quantitated in the presence or absence of IGF-1 as the number of cells migrating across the wound edge. Proliferation was assessed by thymidine incorporation. IGF-1-enhanced epithelial cell migration by 2- to 2.5-fold after 12- and 24-hour treatment, respectively, the first step involved in gastrointestinal wound healing. Cell proliferation was significantly stimulated by IGF-1 as well. In addition, expression of transforming growth factor-β (TGF-β) mRNA was significantly enhanced in the wounded monolayers treated with IGF-1. IGF-1 receptor mRNA was found to be detectable throughtout the gastrointestinal mucosa and in the intestinal epithelial cells. In conclusion, these findings suggest that IGF-1 plays an important role in reconstitution of intestinal epithelial integrity after mucosal injury. (Journal of Parenteral and Enteral Nutrition23: S89-S92, 1999)</abstract><cop>Thousand Oaks, CA</cop><pub>SAGE Publications</pub><pmid>10483904</pmid><doi>10.1177/014860719902300522</doi><tpages>4</tpages></addata></record> |
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| ispartof | JPEN. Journal of parenteral and enteral nutrition, 1999-09, Vol.23 (5_suppl), p.S89-S92 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Animals Cell Division - drug effects Cell Line Humans Insulin-Like Growth Factor I - physiology Intestinal Mucosa - drug effects Intestines - injuries RNA, Messenger - isolation & purification Wound Healing - drug effects |
| title | Insulin-Like Growth Factor-1 Modulation of Intestinal Epithelial Cell Restitution |
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