Insulin-Like Growth Factor-1 Modulation of Intestinal Epithelial Cell Restitution

After superficial intestinal injury, the mucosal integrity is reestablished by rapid migration of epithelial cells from the adjacent area in a process called restitution. Our previous study suggested that growth hormone improves intestinal healing in an experimental small bowel ulceration, mediated...

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Veröffentlicht in:JPEN. Journal of parenteral and enteral nutrition 1999-09, Vol.23 (5_suppl), p.S89-S92
Hauptverfasser: Kai Chen, Nezu, Riichiro, Wasa, Masafumi, Sando, Kinya, Kamata, Shinkichi, Takagi, Yoji, Okada, Akira
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Sprache:eng
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Zusammenfassung:After superficial intestinal injury, the mucosal integrity is reestablished by rapid migration of epithelial cells from the adjacent area in a process called restitution. Our previous study suggested that growth hormone improves intestinal healing in an experimental small bowel ulceration, mediated by insulin-like growth factor-1 (IGF-1). The aim of the present study was to assess the role of IGF-1 in mucosal epithelial restitution using an in vitro epithelial wound model. Wounds were established in confluent monolayers of the intestinal cell line, IEC-6. Migration was quantitated in the presence or absence of IGF-1 as the number of cells migrating across the wound edge. Proliferation was assessed by thymidine incorporation. IGF-1-enhanced epithelial cell migration by 2- to 2.5-fold after 12- and 24-hour treatment, respectively, the first step involved in gastrointestinal wound healing. Cell proliferation was significantly stimulated by IGF-1 as well. In addition, expression of transforming growth factor-β (TGF-β) mRNA was significantly enhanced in the wounded monolayers treated with IGF-1. IGF-1 receptor mRNA was found to be detectable throughtout the gastrointestinal mucosa and in the intestinal epithelial cells. In conclusion, these findings suggest that IGF-1 plays an important role in reconstitution of intestinal epithelial integrity after mucosal injury. (Journal of Parenteral and Enteral Nutrition23: S89-S92, 1999)
ISSN:0148-6071
1941-2444
DOI:10.1177/014860719902300522