Progesterone-Induced Blocking Factor Inhibits Degranulation of Natural Killer Cells
PROBLEM: During the first trimester of pregnancy, nonclassical (CD3—, CD56+, CD16—, perforin [P]bright +) natural killer (NK) cells comprise the major decidual lymphocyte population. These cells, in spite of their high perforin content, exert a low cytolytic activity. Peripheral blood lymphocytes of...
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Veröffentlicht in: | American journal of reproductive immunology (1989) 1999-08, Vol.42 (2), p.71-75 |
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Zusammenfassung: | PROBLEM: During the first trimester of pregnancy, nonclassical (CD3—, CD56+, CD16—, perforin [P]bright +) natural killer (NK) cells comprise the major decidual lymphocyte population. These cells, in spite of their high perforin content, exert a low cytolytic activity. Peripheral blood lymphocytes of healthy pregnant women produce progesterone‐induced blocking factor (PIBF), which inhibits NK activity. PIBF‐producing cells are likely to be present in decidua and might contribute to low decidual NK activity.
METHOD OF STUDY: Decidual cells obtained from elective pregnancy termination were double labeled for CD56 and PIBF. We tested the effect of PIBF on perforin liberation by activated peripheral blood NK cells.
RESULTS: Sixty percent of decidual lymphocytes were CD56 + and expressed PIBF at the same time. PIBF‐treated and untreated peripheral blood NK cells were incubated with K‐562 cells, and perforin content of target conjugated NK cells was detected with immunocytochemistry. PIBF treatment of peripheral blood lymphocytes significantly reduced lysis of K‐562 cells. Among target bound lymphocytes in PIBF‐treated samples, we found a significantly (P < 0.01) higher rate of P+ cells than in untreated samples.
CONCLUSIONS: These data suggest that PIBF inhibits cytotoxicity of NK cells via a block of degranulation, and since decidual NK cells are PIBF+, it cannot be ruled out that this effect of PIBF contributes to low decidual NK activity. |
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ISSN: | 1046-7408 8755-8920 1600-0897 |
DOI: | 10.1111/j.1600-0897.1999.tb00468.x |