Empirical evaluation of preservation methods for faecal DNA

We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free‐ranging baboons showed that amplification success was dependent on preservation method, PCR‐product size, and whether nuclear or mitochondrial...

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Veröffentlicht in:	Molecular ecology 1998-10, Vol.7 (10), p.1423-1428
Hauptverfasser:	FRANTZEN, M. A. J., SILK, J. B., FERGUSON, J. W. H., WAYNE, R. K., KOHN, M. H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals apolipoprotein E Base Sequence cytochrome c oxidase II DNA - genetics DNA - isolation & purification DNA preservation DNA Primers - genetics DNA, Mitochondrial - genetics DNA, Mitochondrial - isolation & purification Ecosystem Electron Transport Complex IV - genetics Evaluation Studies as Topic Feces - chemistry Genetics, Population Genotype molecular scatology noninvasive sampling Papio - genetics Papio cynocephalus ursinus Polymerase Chain Reaction Preservation, Biological - methods Solutions
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Beschreibung
Zusammenfassung:	We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free‐ranging baboons showed that amplification success was dependent on preservation method, PCR‐product size, and whether nuclear or mitochondrial DNA was assayed. Storage in a DMSO/EDTA/Tris/salt solution (DETs) was most effective for preserving nuclear DNA, but storage in 70% ethanol, freezing at –20°C and drying performed approximately equally well for mitochondrial DNA and short (
ISSN:	0962-1083 1365-294X
DOI:	10.1046/j.1365-294x.1998.00449.x