Insect cell line authentication by denaturing gradient gel electrophoresis

We have developed a procedure based on the sequence variation in the mitochondrial 16S rRNA gene that uses DGGE for authentication of insect cell lines. Sequence variation in this gene has been used to identify and classify organisms and here it is applied to identify and characterize mosquito cell...

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Veröffentlicht in:	In vitro cellular & developmental biology. Animal 1998-10, Vol.34 (9), p.665-667
Hauptverfasser:	Kshirsagar, S.G, Patole, M.S, Shouche, Y.S
Format:	Artikel
Sprache:	eng
Schlagworte:	Aedes Aedes - cytology Aedes - embryology Aedes aegypti Aedes albopictus aedes w-albus Animals Anopheles Anopheles - cytology Anopheles stephensi Base Sequence Cell Culture Techniques - standards Cell Line Cell lines Culex - cytology culex bitaeniorhynchus Culicidae DNA DNA Primers Electrophoresis Electrophoresis, Polyacrylamide Gel - methods Gels genes genetic variation identification Insect development Insect genetics Insect vectors Larvae Mosquitos Polymerase Chain Reaction ribosomal DNA ribosomal RNA Scientific Reports Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	We have developed a procedure based on the sequence variation in the mitochondrial 16S rRNA gene that uses DGGE for authentication of insect cell lines. Sequence variation in this gene has been used to identify and classify organisms and here it is applied to identify and characterize mosquito cell lines.
ISSN:	1071-2690 1543-706X
DOI:	10.1007/s11626-998-0058-1