Typing Listeria monocytogenes by random amplified polymorphic DNA (RAPD) fingerprinting

Twenty epidemiologically unrelated Listeria monocytogenes strains isolated from different animals, locations and on different dates in Japan were classified into 18 types by the random amplified polymorphic DNA (RAPD) fingerprinting technique with four primers. Further, seven epidemiologically relat...

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Veröffentlicht in:	Journal of Veterinary Medical Science 1999/07/25, Vol.61(7), pp.857-860
Hauptverfasser:	Yoshida, T. (Nagano-ken. Research Inst. for Health and Pollution (Japan)), Takeuchi, M, Sato, M, Hirai, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Wild Bacterial Typing Techniques BETAIL Cattle Chickens Deer DNA FINGERPRINTING DNA Fingerprinting - methods EMPREINTE ADN Female GANADO Gastrointestinal Contents - microbiology HUELLAS GENETICAS ADN Japan LISTERIA MONOCYTOGENES Listeria monocytogenes - classification Listeria monocytogenes - genetics Listeria monocytogenes - isolation & purification LIVESTOCK Meat - microbiology Milk - microbiology PCR Poultry Random Amplified Polymorphic DNA Technique RAPD RAPD-PCR Swine typing
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Zusammenfassung:	Twenty epidemiologically unrelated Listeria monocytogenes strains isolated from different animals, locations and on different dates in Japan were classified into 18 types by the random amplified polymorphic DNA (RAPD) fingerprinting technique with four primers. Further, seven epidemiologically related L. monocytogenes strains isolated from raw milk and a bulk tank on a dairy farm represented the same RAPD type suggesting that they were all of the same origin. Therefore, RAPD-polymerase chain reaction (PCR) analysis, which is rapid, simple and inexpensive to perform, can be used in surveys as a convenient epidemiological technique.
ISSN:	0916-7250 1347-7439
DOI:	10.1292/jvms.61.857