Molecular cloning of haploid germ cell-specific tektin cDNA and analysis of the protein in mouse testis

Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules. We report here the molecular cloning of a new member of the tektin family, tektin-t, identified from a mouse haploid germ cell-specific cDNA library. Tektin-t mRNA encodes a protein of...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	FEBS letters 1999-08, Vol.456 (2), p.315-321
Hauptverfasser:	Iguchi, Naoko, Tanaka, Hiromitsu, Fujii, Takayuki, Tamura, Kazutoshi, Kaneko, Yoshioki, Nojima, Hiroshi, Nishimune, Yoshitake
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence cDNA library Cloning, Molecular DNA, Complementary - genetics Female Humans Immunohistochemistry Male Mice Microtubule Proteins - analysis Microtubule Proteins - genetics Microtubule Proteins - metabolism Molecular Sequence Data Mouse RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid Sperm Sperm Tail - metabolism Spermatozoa - metabolism Tektin Testis Testis - metabolism Tissue Distribution
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules. We report here the molecular cloning of a new member of the tektin family, tektin-t, identified from a mouse haploid germ cell-specific cDNA library. Tektin-t mRNA encodes a protein of 430 deduced amino acids possessing RSNVELCRD, the conserved sequence of tektin family proteins. Western blotting showed a single band having a molecular weight of 86 kDa in the mouse testis. Immunohistochemistry of the testis showed that tektin-t is localized in the flagella of elongating spermatids from developmental step 15 to maturity.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/S0014-5793(99)00967-9