Role of sonic hedgehog in branchiomotor neuron induction in zebrafish
The role of zebrafish hedgehog genes in branchiomotor neuron development was analyzed by examining mutations that affect the expression of the hedgehog genes and by overexpressing these genes in embryos. In cyclops mutants, reduction in sonic hedgehog ( shh) expression, and elimination of tiggy-wink...
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Veröffentlicht in: | Mechanisms of development 1998-08, Vol.76 (1), p.101-115 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The role of zebrafish
hedgehog genes in branchiomotor neuron development was analyzed by examining mutations that affect the expression of the
hedgehog genes and by overexpressing these genes in embryos. In
cyclops mutants, reduction in
sonic hedgehog (
shh) expression, and elimination of
tiggy-winkle hedgehog (
twhh) expression, correlated with reductions in branchiomotor neuron populations. Furthermore, branchiomotor neurons were restored in
cyclops mutants when
shh or
twhh was overexpressed. These results suggest that Shh and/or Twhh play an important role in the induction of branchiomotor neurons in vivo. In
sonic-you (
syu) mutants, where Shh activity was reduced or eliminated due to mutations in
shh, branchiomotor neurons were reduced in number in a rhombomere-specific fashion, but never eliminated. Similarly, spinal motor neurons were reduced, but not eliminated, in
syu mutants. These results demonstrate that Shh is not solely responsible for inducing branchiomotor and spinal motor neurons, and suggest that Shh and Twhh may function as partially redundant signals for motor neuron induction in zebrafish. |
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ISSN: | 0925-4773 1872-6356 |
DOI: | 10.1016/S0925-4773(98)00101-4 |