Gating kinetics of E. coli poly-3-hydroxybutyrate/polyphosphate channels in planar bilayer membranes

Nonproteinaceous calcium channel complexes from Escherichia coli, composed of poly-(R)-3-hydroxybutyrate (PHB) and inorganic polyphosphate (polyP), exhibit two distinct gating modes (modes 1 and 2) in planar lipid bilayers. Here we report the kinetic characterization of the channel in mode 2, a mode...

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Veröffentlicht in:	The Journal of membrane biology 1999-07, Vol.170 (2), p.135-145
Hauptverfasser:	Das, S, Reusch, R N
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Calcium Channels - metabolism Calcium Channels - physiology Electric Conductivity Escherichia coli Hydroxybutyrates - metabolism Ion Channel Gating - physiology Kinetics Lipid Bilayers - metabolism Patch-Clamp Techniques Polyesters - metabolism Polyphosphates - metabolism
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Zusammenfassung:	Nonproteinaceous calcium channel complexes from Escherichia coli, composed of poly-(R)-3-hydroxybutyrate (PHB) and inorganic polyphosphate (polyP), exhibit two distinct gating modes (modes 1 and 2) in planar lipid bilayers. Here we report the kinetic characterization of the channel in mode 2, a mode characterized by two well-defined conductance levels, a fully open state (87 +/- 3 pS), and a major subconductance state (56 +/- 2 pS). Other subconductance states and full closures are rare (
ISSN:	0022-2631 1432-1424
DOI:	10.1007/s002329900543