The detection of human β 2-microglobulin by grating coupler immunosensor with three dimensional antibody networks

Immunosensors for the detection of human β 2-rnicroglobulin (B2M) were prepared by immobilisation of covalently crosslinked assemblies containing various numbers of molecular layers of monoclonal antibody against B2M (anti-B2M) on the surface of a Ta 2O 5 grating coupler sensor. The immobilisation p...

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Veröffentlicht in:Biosensors & bioelectronics 1999-04, Vol.14 (4), p.363-368
Hauptverfasser: Brynda, E., Houska, M., Brandenburg, A., Wikerstal, A., Škvor, J.
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Sprache:eng
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Zusammenfassung:Immunosensors for the detection of human β 2-rnicroglobulin (B2M) were prepared by immobilisation of covalently crosslinked assemblies containing various numbers of molecular layers of monoclonal antibody against B2M (anti-B2M) on the surface of a Ta 2O 5 grating coupler sensor. The immobilisation procedure consisted of repeated successive adsorption of anti-B2M and dextran sulfate (DS) followed by glutaraldehyde (GA) crosslinking of anti-B2M and washing out DS. The flexibility of the resulting anti-B2M networks was evaluated from the sensor response to the reversible expansion and contraction of the networks induced by changing pH of the ambient solution. A decreased GA concentration and the use of a higher-molecular-mass DS increased the network flexibility. The sensor sensitivity to B2M increased with increasing flexibility of the antibody networks and with increasing number of anti-B2M molecular layers, indicating that B2M can penetrate inside the antibody network.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(99)00013-5