Engineering of Thermus thermophilus Cytochrome c₅₅₂: Thermally Tolerant Artificial Peroxidase

Engineering of Thermus thermophilus Cytochrome c₅₅₂: Thermally Tolerant Artificial Peroxidase

Customizing enzymes: We demonstrate that cytochrome c552 (Cyt c552) from Thermus thermophilus HB8 can be transformed into a thermally tolerant peroxidase by a design process modeled on the catalytic mechanism of peroxidases. At temperatures above 50 °C, the enzymatic activity of the engineered Cyt c...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chembiochem : a European journal of chemical biology 2008-12, Vol.9 (18), p.2954-2957
Hauptverfasser: Nakajima, Hiroshi, Ichikawa, Yusuke, Satake, Yuh, Takatani, Nobuyuki, Manna, Soumen Kanti, Rajbongshi, Jitumani, Mazumdar, Shyamalava, Watanabe, Yoshihito
Format: Artikel
Sprache:eng
Schlagworte:
artificial metalloenzymes
Circular Dichroism
Cytochrome c Group - chemistry
Cytochrome c Group - genetics
cytochromes
Kinetics
Models, Molecular
peroxidases
Peroxidases - chemistry
Peroxidases - genetics
Protein Engineering
Temperature
Thermus thermophilus
Thermus thermophilus - enzymology
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Customizing enzymes: We demonstrate that cytochrome c552 (Cyt c552) from Thermus thermophilus HB8 can be transformed into a thermally tolerant peroxidase by a design process modeled on the catalytic mechanism of peroxidases. At temperatures above 50 °C, the enzymatic activity of the engineered Cyt c552 surpasses that of a myoglobin variant that is known to exhibit the highest activity among artificial peroxidases.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200800599