Leginon: a system for fully automated acquisition of 1000 electron micrographs a day
We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as...
Gespeichert in:
Veröffentlicht in: | Ultramicroscopy 1999-07, Vol.77 (3), p.153-161 |
---|---|
Hauptverfasser: | , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 161 |
---|---|
container_issue | 3 |
container_start_page | 153 |
container_title | Ultramicroscopy |
container_volume | 77 |
creator | Potter, C.S Chu, H Frey, B Green, C Kisseberth, N Madden, T.J Miller, K.L Nahrstedt, K Pulokas, J Reilein, A Tcheng, D Weber, D Carragher, B |
description | We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs. |
doi_str_mv | 10.1016/S0304-3991(99)00043-1 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69891887</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0304399199000431</els_id><sourcerecordid>69891887</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-2bb1ba228e037fdafecb3f943371761eb125f9fa405989313f1e035642d4a5483</originalsourceid><addsrcrecordid>eNqFkE1r3DAQhkVISbbb_IQEHUJpD040lvyhXkpZkjawkEO2ZyHLo62CbW0ku7D_PtoPmtx6Ghie953hIeQS2A0wKG-fGGci41LCFym_MsYEz-CEzKCuZJZXOT8ls3_IOfkY43OCgIn6jJynwUrg-Yyslrh2gx--UU3jNo7YU-sDtVPXbameRt_rEVuqzcvkohudH6i3FFIVxQ7NGNKidyb4ddCbPzG1tHr7iXywuot4cZxz8vv-brX4lS0ffz4sfiwzUzA-ZnnTQKPzvEbGK9tqi6bhVgrOK6hKwAbywkqrBStkLTlwC4ksSpG3Qhei5nPy-dC7Cf5lwjiq3kWDXacH9FNUZYpBXVcJLA5gejTGgFZtgut12CpgaqdT7XWqnSslpdrrVJByV8cDU9Nj-y518JeA6yOgo9GdDXowLr5xdSkEsIR9P2CYbPx1GFQ0DgeDrQtJomq9-88nr7RYj_8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69891887</pqid></control><display><type>article</type><title>Leginon: a system for fully automated acquisition of 1000 electron micrographs a day</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Potter, C.S ; Chu, H ; Frey, B ; Green, C ; Kisseberth, N ; Madden, T.J ; Miller, K.L ; Nahrstedt, K ; Pulokas, J ; Reilein, A ; Tcheng, D ; Weber, D ; Carragher, B</creator><creatorcontrib>Potter, C.S ; Chu, H ; Frey, B ; Green, C ; Kisseberth, N ; Madden, T.J ; Miller, K.L ; Nahrstedt, K ; Pulokas, J ; Reilein, A ; Tcheng, D ; Weber, D ; Carragher, B</creatorcontrib><description>We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.</description><identifier>ISSN: 0304-3991</identifier><identifier>EISSN: 1879-2723</identifier><identifier>DOI: 10.1016/S0304-3991(99)00043-1</identifier><identifier>PMID: 10406132</identifier><identifier>CODEN: ULTRD6</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Automated data acquisition ; Automation - methods ; Biological and medical sciences ; Computers in experimental physics ; Condensed matter: structure, mechanical and thermal properties ; Electron, ion, and scanning probe microscopy ; Electron, positron and ion microscopes, electron diffractometers and related techniques ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation technics, apparatus ; Humans ; Image processing ; Instrument control ; Instruments, apparatus, components and techniques common to several branches of physics and astronomy ; Microscopic methods ; Microscopy, Electron - methods ; Physics ; Scanning tunneling and atomic force microscopy ; Structure of solids and liquids; crystallography ; Three-dimensional electron microscopy ; Tissues, organs and organisms biophysics ; Transmission, reflection and scanning electron microscopy(including ebic)</subject><ispartof>Ultramicroscopy, 1999-07, Vol.77 (3), p.153-161</ispartof><rights>1999 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-2bb1ba228e037fdafecb3f943371761eb125f9fa405989313f1e035642d4a5483</citedby><cites>FETCH-LOGICAL-c503t-2bb1ba228e037fdafecb3f943371761eb125f9fa405989313f1e035642d4a5483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-3991(99)00043-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1864410$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10406132$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Potter, C.S</creatorcontrib><creatorcontrib>Chu, H</creatorcontrib><creatorcontrib>Frey, B</creatorcontrib><creatorcontrib>Green, C</creatorcontrib><creatorcontrib>Kisseberth, N</creatorcontrib><creatorcontrib>Madden, T.J</creatorcontrib><creatorcontrib>Miller, K.L</creatorcontrib><creatorcontrib>Nahrstedt, K</creatorcontrib><creatorcontrib>Pulokas, J</creatorcontrib><creatorcontrib>Reilein, A</creatorcontrib><creatorcontrib>Tcheng, D</creatorcontrib><creatorcontrib>Weber, D</creatorcontrib><creatorcontrib>Carragher, B</creatorcontrib><title>Leginon: a system for fully automated acquisition of 1000 electron micrographs a day</title><title>Ultramicroscopy</title><addtitle>Ultramicroscopy</addtitle><description>We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.</description><subject>Automated data acquisition</subject><subject>Automation - methods</subject><subject>Biological and medical sciences</subject><subject>Computers in experimental physics</subject><subject>Condensed matter: structure, mechanical and thermal properties</subject><subject>Electron, ion, and scanning probe microscopy</subject><subject>Electron, positron and ion microscopes, electron diffractometers and related techniques</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation technics, apparatus</subject><subject>Humans</subject><subject>Image processing</subject><subject>Instrument control</subject><subject>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</subject><subject>Microscopic methods</subject><subject>Microscopy, Electron - methods</subject><subject>Physics</subject><subject>Scanning tunneling and atomic force microscopy</subject><subject>Structure of solids and liquids; crystallography</subject><subject>Three-dimensional electron microscopy</subject><subject>Tissues, organs and organisms biophysics</subject><subject>Transmission, reflection and scanning electron microscopy(including ebic)</subject><issn>0304-3991</issn><issn>1879-2723</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVISbbb_IQEHUJpD040lvyhXkpZkjawkEO2ZyHLo62CbW0ku7D_PtoPmtx6Ghie953hIeQS2A0wKG-fGGci41LCFym_MsYEz-CEzKCuZJZXOT8ls3_IOfkY43OCgIn6jJynwUrg-Yyslrh2gx--UU3jNo7YU-sDtVPXbameRt_rEVuqzcvkohudH6i3FFIVxQ7NGNKidyb4ddCbPzG1tHr7iXywuot4cZxz8vv-brX4lS0ffz4sfiwzUzA-ZnnTQKPzvEbGK9tqi6bhVgrOK6hKwAbywkqrBStkLTlwC4ksSpG3Qhei5nPy-dC7Cf5lwjiq3kWDXacH9FNUZYpBXVcJLA5gejTGgFZtgut12CpgaqdT7XWqnSslpdrrVJByV8cDU9Nj-y518JeA6yOgo9GdDXowLr5xdSkEsIR9P2CYbPx1GFQ0DgeDrQtJomq9-88nr7RYj_8</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Potter, C.S</creator><creator>Chu, H</creator><creator>Frey, B</creator><creator>Green, C</creator><creator>Kisseberth, N</creator><creator>Madden, T.J</creator><creator>Miller, K.L</creator><creator>Nahrstedt, K</creator><creator>Pulokas, J</creator><creator>Reilein, A</creator><creator>Tcheng, D</creator><creator>Weber, D</creator><creator>Carragher, B</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990701</creationdate><title>Leginon: a system for fully automated acquisition of 1000 electron micrographs a day</title><author>Potter, C.S ; Chu, H ; Frey, B ; Green, C ; Kisseberth, N ; Madden, T.J ; Miller, K.L ; Nahrstedt, K ; Pulokas, J ; Reilein, A ; Tcheng, D ; Weber, D ; Carragher, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-2bb1ba228e037fdafecb3f943371761eb125f9fa405989313f1e035642d4a5483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Automated data acquisition</topic><topic>Automation - methods</topic><topic>Biological and medical sciences</topic><topic>Computers in experimental physics</topic><topic>Condensed matter: structure, mechanical and thermal properties</topic><topic>Electron, ion, and scanning probe microscopy</topic><topic>Electron, positron and ion microscopes, electron diffractometers and related techniques</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation technics, apparatus</topic><topic>Humans</topic><topic>Image processing</topic><topic>Instrument control</topic><topic>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</topic><topic>Microscopic methods</topic><topic>Microscopy, Electron - methods</topic><topic>Physics</topic><topic>Scanning tunneling and atomic force microscopy</topic><topic>Structure of solids and liquids; crystallography</topic><topic>Three-dimensional electron microscopy</topic><topic>Tissues, organs and organisms biophysics</topic><topic>Transmission, reflection and scanning electron microscopy(including ebic)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Potter, C.S</creatorcontrib><creatorcontrib>Chu, H</creatorcontrib><creatorcontrib>Frey, B</creatorcontrib><creatorcontrib>Green, C</creatorcontrib><creatorcontrib>Kisseberth, N</creatorcontrib><creatorcontrib>Madden, T.J</creatorcontrib><creatorcontrib>Miller, K.L</creatorcontrib><creatorcontrib>Nahrstedt, K</creatorcontrib><creatorcontrib>Pulokas, J</creatorcontrib><creatorcontrib>Reilein, A</creatorcontrib><creatorcontrib>Tcheng, D</creatorcontrib><creatorcontrib>Weber, D</creatorcontrib><creatorcontrib>Carragher, B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Ultramicroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Potter, C.S</au><au>Chu, H</au><au>Frey, B</au><au>Green, C</au><au>Kisseberth, N</au><au>Madden, T.J</au><au>Miller, K.L</au><au>Nahrstedt, K</au><au>Pulokas, J</au><au>Reilein, A</au><au>Tcheng, D</au><au>Weber, D</au><au>Carragher, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leginon: a system for fully automated acquisition of 1000 electron micrographs a day</atitle><jtitle>Ultramicroscopy</jtitle><addtitle>Ultramicroscopy</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>77</volume><issue>3</issue><spage>153</spage><epage>161</epage><pages>153-161</pages><issn>0304-3991</issn><eissn>1879-2723</eissn><coden>ULTRD6</coden><abstract>We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10406132</pmid><doi>10.1016/S0304-3991(99)00043-1</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0304-3991 |
ispartof | Ultramicroscopy, 1999-07, Vol.77 (3), p.153-161 |
issn | 0304-3991 1879-2723 |
language | eng |
recordid | cdi_proquest_miscellaneous_69891887 |
source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
subjects | Automated data acquisition Automation - methods Biological and medical sciences Computers in experimental physics Condensed matter: structure, mechanical and thermal properties Electron, ion, and scanning probe microscopy Electron, positron and ion microscopes, electron diffractometers and related techniques Exact sciences and technology Fundamental and applied biological sciences. Psychology General aspects, investigation technics, apparatus Humans Image processing Instrument control Instruments, apparatus, components and techniques common to several branches of physics and astronomy Microscopic methods Microscopy, Electron - methods Physics Scanning tunneling and atomic force microscopy Structure of solids and liquids crystallography Three-dimensional electron microscopy Tissues, organs and organisms biophysics Transmission, reflection and scanning electron microscopy(including ebic) |
title | Leginon: a system for fully automated acquisition of 1000 electron micrographs a day |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T13%3A58%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Leginon:%20a%20system%20for%20fully%20automated%20acquisition%20of%201000%20electron%20micrographs%20a%20day&rft.jtitle=Ultramicroscopy&rft.au=Potter,%20C.S&rft.date=1999-07-01&rft.volume=77&rft.issue=3&rft.spage=153&rft.epage=161&rft.pages=153-161&rft.issn=0304-3991&rft.eissn=1879-2723&rft.coden=ULTRD6&rft_id=info:doi/10.1016/S0304-3991(99)00043-1&rft_dat=%3Cproquest_cross%3E69891887%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69891887&rft_id=info:pmid/10406132&rft_els_id=S0304399199000431&rfr_iscdi=true |