Leginon: a system for fully automated acquisition of 1000 electron micrographs a day

We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Ultramicroscopy 1999-07, Vol.77 (3), p.153-161
Hauptverfasser: Potter, C.S, Chu, H, Frey, B, Green, C, Kisseberth, N, Madden, T.J, Miller, K.L, Nahrstedt, K, Pulokas, J, Reilein, A, Tcheng, D, Weber, D, Carragher, B
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 161
container_issue 3
container_start_page 153
container_title Ultramicroscopy
container_volume 77
creator Potter, C.S
Chu, H
Frey, B
Green, C
Kisseberth, N
Madden, T.J
Miller, K.L
Nahrstedt, K
Pulokas, J
Reilein, A
Tcheng, D
Weber, D
Carragher, B
description We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.
doi_str_mv 10.1016/S0304-3991(99)00043-1
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69891887</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0304399199000431</els_id><sourcerecordid>69891887</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-2bb1ba228e037fdafecb3f943371761eb125f9fa405989313f1e035642d4a5483</originalsourceid><addsrcrecordid>eNqFkE1r3DAQhkVISbbb_IQEHUJpD040lvyhXkpZkjawkEO2ZyHLo62CbW0ku7D_PtoPmtx6Ghie953hIeQS2A0wKG-fGGci41LCFym_MsYEz-CEzKCuZJZXOT8ls3_IOfkY43OCgIn6jJynwUrg-Yyslrh2gx--UU3jNo7YU-sDtVPXbameRt_rEVuqzcvkohudH6i3FFIVxQ7NGNKidyb4ddCbPzG1tHr7iXywuot4cZxz8vv-brX4lS0ffz4sfiwzUzA-ZnnTQKPzvEbGK9tqi6bhVgrOK6hKwAbywkqrBStkLTlwC4ksSpG3Qhei5nPy-dC7Cf5lwjiq3kWDXacH9FNUZYpBXVcJLA5gejTGgFZtgut12CpgaqdT7XWqnSslpdrrVJByV8cDU9Nj-y518JeA6yOgo9GdDXowLr5xdSkEsIR9P2CYbPx1GFQ0DgeDrQtJomq9-88nr7RYj_8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69891887</pqid></control><display><type>article</type><title>Leginon: a system for fully automated acquisition of 1000 electron micrographs a day</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Potter, C.S ; Chu, H ; Frey, B ; Green, C ; Kisseberth, N ; Madden, T.J ; Miller, K.L ; Nahrstedt, K ; Pulokas, J ; Reilein, A ; Tcheng, D ; Weber, D ; Carragher, B</creator><creatorcontrib>Potter, C.S ; Chu, H ; Frey, B ; Green, C ; Kisseberth, N ; Madden, T.J ; Miller, K.L ; Nahrstedt, K ; Pulokas, J ; Reilein, A ; Tcheng, D ; Weber, D ; Carragher, B</creatorcontrib><description>We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.</description><identifier>ISSN: 0304-3991</identifier><identifier>EISSN: 1879-2723</identifier><identifier>DOI: 10.1016/S0304-3991(99)00043-1</identifier><identifier>PMID: 10406132</identifier><identifier>CODEN: ULTRD6</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Automated data acquisition ; Automation - methods ; Biological and medical sciences ; Computers in experimental physics ; Condensed matter: structure, mechanical and thermal properties ; Electron, ion, and scanning probe microscopy ; Electron, positron and ion microscopes, electron diffractometers and related techniques ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation technics, apparatus ; Humans ; Image processing ; Instrument control ; Instruments, apparatus, components and techniques common to several branches of physics and astronomy ; Microscopic methods ; Microscopy, Electron - methods ; Physics ; Scanning tunneling and atomic force microscopy ; Structure of solids and liquids; crystallography ; Three-dimensional electron microscopy ; Tissues, organs and organisms biophysics ; Transmission, reflection and scanning electron microscopy(including ebic)</subject><ispartof>Ultramicroscopy, 1999-07, Vol.77 (3), p.153-161</ispartof><rights>1999 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-2bb1ba228e037fdafecb3f943371761eb125f9fa405989313f1e035642d4a5483</citedby><cites>FETCH-LOGICAL-c503t-2bb1ba228e037fdafecb3f943371761eb125f9fa405989313f1e035642d4a5483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-3991(99)00043-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=1864410$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10406132$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Potter, C.S</creatorcontrib><creatorcontrib>Chu, H</creatorcontrib><creatorcontrib>Frey, B</creatorcontrib><creatorcontrib>Green, C</creatorcontrib><creatorcontrib>Kisseberth, N</creatorcontrib><creatorcontrib>Madden, T.J</creatorcontrib><creatorcontrib>Miller, K.L</creatorcontrib><creatorcontrib>Nahrstedt, K</creatorcontrib><creatorcontrib>Pulokas, J</creatorcontrib><creatorcontrib>Reilein, A</creatorcontrib><creatorcontrib>Tcheng, D</creatorcontrib><creatorcontrib>Weber, D</creatorcontrib><creatorcontrib>Carragher, B</creatorcontrib><title>Leginon: a system for fully automated acquisition of 1000 electron micrographs a day</title><title>Ultramicroscopy</title><addtitle>Ultramicroscopy</addtitle><description>We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.</description><subject>Automated data acquisition</subject><subject>Automation - methods</subject><subject>Biological and medical sciences</subject><subject>Computers in experimental physics</subject><subject>Condensed matter: structure, mechanical and thermal properties</subject><subject>Electron, ion, and scanning probe microscopy</subject><subject>Electron, positron and ion microscopes, electron diffractometers and related techniques</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation technics, apparatus</subject><subject>Humans</subject><subject>Image processing</subject><subject>Instrument control</subject><subject>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</subject><subject>Microscopic methods</subject><subject>Microscopy, Electron - methods</subject><subject>Physics</subject><subject>Scanning tunneling and atomic force microscopy</subject><subject>Structure of solids and liquids; crystallography</subject><subject>Three-dimensional electron microscopy</subject><subject>Tissues, organs and organisms biophysics</subject><subject>Transmission, reflection and scanning electron microscopy(including ebic)</subject><issn>0304-3991</issn><issn>1879-2723</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVISbbb_IQEHUJpD040lvyhXkpZkjawkEO2ZyHLo62CbW0ku7D_PtoPmtx6Ghie953hIeQS2A0wKG-fGGci41LCFym_MsYEz-CEzKCuZJZXOT8ls3_IOfkY43OCgIn6jJynwUrg-Yyslrh2gx--UU3jNo7YU-sDtVPXbameRt_rEVuqzcvkohudH6i3FFIVxQ7NGNKidyb4ddCbPzG1tHr7iXywuot4cZxz8vv-brX4lS0ffz4sfiwzUzA-ZnnTQKPzvEbGK9tqi6bhVgrOK6hKwAbywkqrBStkLTlwC4ksSpG3Qhei5nPy-dC7Cf5lwjiq3kWDXacH9FNUZYpBXVcJLA5gejTGgFZtgut12CpgaqdT7XWqnSslpdrrVJByV8cDU9Nj-y518JeA6yOgo9GdDXowLr5xdSkEsIR9P2CYbPx1GFQ0DgeDrQtJomq9-88nr7RYj_8</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Potter, C.S</creator><creator>Chu, H</creator><creator>Frey, B</creator><creator>Green, C</creator><creator>Kisseberth, N</creator><creator>Madden, T.J</creator><creator>Miller, K.L</creator><creator>Nahrstedt, K</creator><creator>Pulokas, J</creator><creator>Reilein, A</creator><creator>Tcheng, D</creator><creator>Weber, D</creator><creator>Carragher, B</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990701</creationdate><title>Leginon: a system for fully automated acquisition of 1000 electron micrographs a day</title><author>Potter, C.S ; Chu, H ; Frey, B ; Green, C ; Kisseberth, N ; Madden, T.J ; Miller, K.L ; Nahrstedt, K ; Pulokas, J ; Reilein, A ; Tcheng, D ; Weber, D ; Carragher, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-2bb1ba228e037fdafecb3f943371761eb125f9fa405989313f1e035642d4a5483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Automated data acquisition</topic><topic>Automation - methods</topic><topic>Biological and medical sciences</topic><topic>Computers in experimental physics</topic><topic>Condensed matter: structure, mechanical and thermal properties</topic><topic>Electron, ion, and scanning probe microscopy</topic><topic>Electron, positron and ion microscopes, electron diffractometers and related techniques</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation technics, apparatus</topic><topic>Humans</topic><topic>Image processing</topic><topic>Instrument control</topic><topic>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</topic><topic>Microscopic methods</topic><topic>Microscopy, Electron - methods</topic><topic>Physics</topic><topic>Scanning tunneling and atomic force microscopy</topic><topic>Structure of solids and liquids; crystallography</topic><topic>Three-dimensional electron microscopy</topic><topic>Tissues, organs and organisms biophysics</topic><topic>Transmission, reflection and scanning electron microscopy(including ebic)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Potter, C.S</creatorcontrib><creatorcontrib>Chu, H</creatorcontrib><creatorcontrib>Frey, B</creatorcontrib><creatorcontrib>Green, C</creatorcontrib><creatorcontrib>Kisseberth, N</creatorcontrib><creatorcontrib>Madden, T.J</creatorcontrib><creatorcontrib>Miller, K.L</creatorcontrib><creatorcontrib>Nahrstedt, K</creatorcontrib><creatorcontrib>Pulokas, J</creatorcontrib><creatorcontrib>Reilein, A</creatorcontrib><creatorcontrib>Tcheng, D</creatorcontrib><creatorcontrib>Weber, D</creatorcontrib><creatorcontrib>Carragher, B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Ultramicroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Potter, C.S</au><au>Chu, H</au><au>Frey, B</au><au>Green, C</au><au>Kisseberth, N</au><au>Madden, T.J</au><au>Miller, K.L</au><au>Nahrstedt, K</au><au>Pulokas, J</au><au>Reilein, A</au><au>Tcheng, D</au><au>Weber, D</au><au>Carragher, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leginon: a system for fully automated acquisition of 1000 electron micrographs a day</atitle><jtitle>Ultramicroscopy</jtitle><addtitle>Ultramicroscopy</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>77</volume><issue>3</issue><spage>153</spage><epage>161</epage><pages>153-161</pages><issn>0304-3991</issn><eissn>1879-2723</eissn><coden>ULTRD6</coden><abstract>We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10406132</pmid><doi>10.1016/S0304-3991(99)00043-1</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0304-3991
ispartof Ultramicroscopy, 1999-07, Vol.77 (3), p.153-161
issn 0304-3991
1879-2723
language eng
recordid cdi_proquest_miscellaneous_69891887
source Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE
subjects Automated data acquisition
Automation - methods
Biological and medical sciences
Computers in experimental physics
Condensed matter: structure, mechanical and thermal properties
Electron, ion, and scanning probe microscopy
Electron, positron and ion microscopes, electron diffractometers and related techniques
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General aspects, investigation technics, apparatus
Humans
Image processing
Instrument control
Instruments, apparatus, components and techniques common to several branches of physics and astronomy
Microscopic methods
Microscopy, Electron - methods
Physics
Scanning tunneling and atomic force microscopy
Structure of solids and liquids
crystallography
Three-dimensional electron microscopy
Tissues, organs and organisms biophysics
Transmission, reflection and scanning electron microscopy(including ebic)
title Leginon: a system for fully automated acquisition of 1000 electron micrographs a day
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T13%3A58%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Leginon:%20a%20system%20for%20fully%20automated%20acquisition%20of%201000%20electron%20micrographs%20a%20day&rft.jtitle=Ultramicroscopy&rft.au=Potter,%20C.S&rft.date=1999-07-01&rft.volume=77&rft.issue=3&rft.spage=153&rft.epage=161&rft.pages=153-161&rft.issn=0304-3991&rft.eissn=1879-2723&rft.coden=ULTRD6&rft_id=info:doi/10.1016/S0304-3991(99)00043-1&rft_dat=%3Cproquest_cross%3E69891887%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69891887&rft_id=info:pmid/10406132&rft_els_id=S0304399199000431&rfr_iscdi=true