Leginon: a system for fully automated acquisition of 1000 electron micrographs a day

We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as...

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Veröffentlicht in:	Ultramicroscopy 1999-07, Vol.77 (3), p.153-161
Hauptverfasser:	Potter, C.S, Chu, H, Frey, B, Green, C, Kisseberth, N, Madden, T.J, Miller, K.L, Nahrstedt, K, Pulokas, J, Reilein, A, Tcheng, D, Weber, D, Carragher, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Automated data acquisition Automation - methods Biological and medical sciences Computers in experimental physics Condensed matter: structure, mechanical and thermal properties Electron, ion, and scanning probe microscopy Electron, positron and ion microscopes, electron diffractometers and related techniques Exact sciences and technology Fundamental and applied biological sciences. Psychology General aspects, investigation technics, apparatus Humans Image processing Instrument control Instruments, apparatus, components and techniques common to several branches of physics and astronomy Microscopic methods Microscopy, Electron - methods Physics Scanning tunneling and atomic force microscopy Structure of solids and liquids crystallography Three-dimensional electron microscopy Tissues, organs and organisms biophysics Transmission, reflection and scanning electron microscopy(including ebic)
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Zusammenfassung:	We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.
ISSN:	0304-3991 1879-2723
DOI:	10.1016/S0304-3991(99)00043-1