Determination of betulinic acid from Orthosiphon stamineus leaf extract in rat plasma

A simple high-performance liquid chromatography (HPLC) method to determine the content of betulinic acid (BA) in rat plasma collected at different times (0-8 h) after oral administration of Orthosiphon stamineus leaf extract was developed. The features of the assay include protein precipitation usin...

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Veröffentlicht in:Pharmazie 2008-11, Vol.63 (11), p.788-790
Hauptverfasser: Akowuah, G. A., Zhari, I.
Format: Artikel
Sprache:eng
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Zusammenfassung:A simple high-performance liquid chromatography (HPLC) method to determine the content of betulinic acid (BA) in rat plasma collected at different times (0-8 h) after oral administration of Orthosiphon stamineus leaf extract was developed. The features of the assay include protein precipitation using acetonitrile and isocratic elution using reverse phase C-18 column with ultraviolet (UV) detection. The recovery of BA from plasma varied from 98.4 to 102.5%. The R.S.D of intra- and inter-day precision from rat plasma ranged from 4.2 to 9.8%. The maximum concentration of BA in the plasma was 1.2 ± 0.3 μg/ml at 1 h after oral administration of the extract.
ISSN:0031-7144
DOI:10.1691/ph.2008.8596