Interference of Linoleic Acid Fraction in Some Receptor Binding Assays
An extract of a suspension culture of Tabernaemontana pandacaqui Poir. was fractionated by centrifugal partition chromatography. Aliquots were tested in an adenosine A1 receptor binding assay. This led to the isolation and identification of linoleic acid, which proved to be a noncompetitive inhibito...
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| Veröffentlicht in: | Journal of natural products (Washington, D.C.) D.C.), 1999-06, Vol.62 (6), p.912-914 |
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| container_end_page | 914 |
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| container_issue | 6 |
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| container_title | Journal of natural products (Washington, D.C.) |
| container_volume | 62 |
| creator | Ingkaninan, K von Frijtag Drabbe Künzel, J. K IJzerman, A. P Verpoorte, R |
| description | An extract of a suspension culture of Tabernaemontana pandacaqui Poir. was fractionated by centrifugal partition chromatography. Aliquots were tested in an adenosine A1 receptor binding assay. This led to the isolation and identification of linoleic acid, which proved to be a noncompetitive inhibitor. This “false positive”effect also extended to some other binding assays. |
| doi_str_mv | 10.1021/np9805490 |
| format | Article |
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This “false positive”effect also extended to some other binding assays.</description><identifier>ISSN: 0163-3864</identifier><identifier>EISSN: 1520-6025</identifier><identifier>DOI: 10.1021/np9805490</identifier><identifier>PMID: 10395519</identifier><identifier>CODEN: JNPRDF</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Aconitum ; aconitum xhenryi ; adenosine ; Animals ; binding sites ; Biological and medical sciences ; Brain - metabolism ; Catharanthus roseus ; cell suspension culture ; Cells, Cultured ; chemical constituents of plants ; chemical structure ; Chromatography, Liquid ; General pharmacology ; In Vitro Techniques ; Indicators and Reagents ; linoleic acid ; Linoleic Acid - isolation & purification ; Linoleic Acid - metabolism ; Linoleic Acid - pharmacology ; Medical sciences ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Purinergic P1 Receptor Antagonists ; Radioligand Assay ; Rats ; receptors ; Receptors, Opioid - metabolism ; Receptors, Purinergic P1 - metabolism ; Rubia tinctorum ; screening ; spectral analysis ; Tabernaemontana ; tabernaemontana pachysiphon ; Tabernaemontana pandacaqui</subject><ispartof>Journal of natural products (Washington, D.C.), 1999-06, Vol.62 (6), p.912-914</ispartof><rights>Copyright © 1999 American Chemical Society and American Society of Pharmacognosy</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a402t-c1ada6890977f8eea52623bd4a941e320e47df2b8f7d61e132935692b30e50e83</citedby><cites>FETCH-LOGICAL-a402t-c1ada6890977f8eea52623bd4a941e320e47df2b8f7d61e132935692b30e50e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/np9805490$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/np9805490$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1891205$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10395519$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ingkaninan, K</creatorcontrib><creatorcontrib>von Frijtag Drabbe Künzel, J. K</creatorcontrib><creatorcontrib>IJzerman, A. P</creatorcontrib><creatorcontrib>Verpoorte, R</creatorcontrib><title>Interference of Linoleic Acid Fraction in Some Receptor Binding Assays</title><title>Journal of natural products (Washington, D.C.)</title><addtitle>J. Nat. Prod</addtitle><description>An extract of a suspension culture of Tabernaemontana pandacaqui Poir. was fractionated by centrifugal partition chromatography. Aliquots were tested in an adenosine A1 receptor binding assay. This led to the isolation and identification of linoleic acid, which proved to be a noncompetitive inhibitor. This “false positive”effect also extended to some other binding assays.</description><subject>Aconitum</subject><subject>aconitum xhenryi</subject><subject>adenosine</subject><subject>Animals</subject><subject>binding sites</subject><subject>Biological and medical sciences</subject><subject>Brain - metabolism</subject><subject>Catharanthus roseus</subject><subject>cell suspension culture</subject><subject>Cells, Cultured</subject><subject>chemical constituents of plants</subject><subject>chemical structure</subject><subject>Chromatography, Liquid</subject><subject>General pharmacology</subject><subject>In Vitro Techniques</subject><subject>Indicators and Reagents</subject><subject>linoleic acid</subject><subject>Linoleic Acid - isolation & purification</subject><subject>Linoleic Acid - metabolism</subject><subject>Linoleic Acid - pharmacology</subject><subject>Medical sciences</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Purinergic P1 Receptor Antagonists</subject><subject>Radioligand Assay</subject><subject>Rats</subject><subject>receptors</subject><subject>Receptors, Opioid - metabolism</subject><subject>Receptors, Purinergic P1 - metabolism</subject><subject>Rubia tinctorum</subject><subject>screening</subject><subject>spectral analysis</subject><subject>Tabernaemontana</subject><subject>tabernaemontana pachysiphon</subject><subject>Tabernaemontana pandacaqui</subject><issn>0163-3864</issn><issn>1520-6025</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0MFu1DAQBmALgei2cOAFwAdA4hAY27ETH5eWpZUigdit1JvldSaVS9be2lmJvj1GWRUOnOYw3_zS_IS8YvCRAWefwl63IGsNT8iCSQ6VAi6fkgUwJSrRqvqEnOZ8BwACtHxOThgILSXTC7K6ChOmARMGhzQOtPMhjugdXTrf01WybvIxUB_oOu6Q_kCH-ykm-tmH3odbuszZPuQX5Nlgx4wvj_OMXK--bM4vq-7b16vzZVfZGvhUOWZ7q1oNummGFtFKrrjY9rXVNUPBAeumH_i2HZpeMWSCayGV5lsBKAFbcUbez7n7FO8PmCez89nhONqA8ZCN0m0jFKsL_DBDl2LOCQezT35n04NhYP6UZh5LK_b1MfSw3WH_j5xbKuDtEdjs7DgkG5zPf12rGQdZWDUznyf89bi26adRjWik2Xxfm013ebO56G5MV_yb2Q82GnubSuT1mgMTwDUrB00R72ZhXTZ38ZBCKfc_H_wGC5KWQA</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Ingkaninan, K</creator><creator>von Frijtag Drabbe Künzel, J. K</creator><creator>IJzerman, A. P</creator><creator>Verpoorte, R</creator><general>American Chemical Society</general><general>American Society of Pharmacognosy</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990601</creationdate><title>Interference of Linoleic Acid Fraction in Some Receptor Binding Assays</title><author>Ingkaninan, K ; von Frijtag Drabbe Künzel, J. K ; IJzerman, A. P ; Verpoorte, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a402t-c1ada6890977f8eea52623bd4a941e320e47df2b8f7d61e132935692b30e50e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Aconitum</topic><topic>aconitum xhenryi</topic><topic>adenosine</topic><topic>Animals</topic><topic>binding sites</topic><topic>Biological and medical sciences</topic><topic>Brain - metabolism</topic><topic>Catharanthus roseus</topic><topic>cell suspension culture</topic><topic>Cells, Cultured</topic><topic>chemical constituents of plants</topic><topic>chemical structure</topic><topic>Chromatography, Liquid</topic><topic>General pharmacology</topic><topic>In Vitro Techniques</topic><topic>Indicators and Reagents</topic><topic>linoleic acid</topic><topic>Linoleic Acid - isolation & purification</topic><topic>Linoleic Acid - metabolism</topic><topic>Linoleic Acid - pharmacology</topic><topic>Medical sciences</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Purinergic P1 Receptor Antagonists</topic><topic>Radioligand Assay</topic><topic>Rats</topic><topic>receptors</topic><topic>Receptors, Opioid - metabolism</topic><topic>Receptors, Purinergic P1 - metabolism</topic><topic>Rubia tinctorum</topic><topic>screening</topic><topic>spectral analysis</topic><topic>Tabernaemontana</topic><topic>tabernaemontana pachysiphon</topic><topic>Tabernaemontana pandacaqui</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ingkaninan, K</creatorcontrib><creatorcontrib>von Frijtag Drabbe Künzel, J. K</creatorcontrib><creatorcontrib>IJzerman, A. 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Prod</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>62</volume><issue>6</issue><spage>912</spage><epage>914</epage><pages>912-914</pages><issn>0163-3864</issn><eissn>1520-6025</eissn><coden>JNPRDF</coden><abstract>An extract of a suspension culture of Tabernaemontana pandacaqui Poir. was fractionated by centrifugal partition chromatography. Aliquots were tested in an adenosine A1 receptor binding assay. This led to the isolation and identification of linoleic acid, which proved to be a noncompetitive inhibitor. This “false positive”effect also extended to some other binding assays.</abstract><cop>Washington, DC</cop><cop>Glendale, AZ</cop><pub>American Chemical Society</pub><pmid>10395519</pmid><doi>10.1021/np9805490</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0163-3864 |
| ispartof | Journal of natural products (Washington, D.C.), 1999-06, Vol.62 (6), p.912-914 |
| issn | 0163-3864 1520-6025 |
| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | Aconitum aconitum xhenryi adenosine Animals binding sites Biological and medical sciences Brain - metabolism Catharanthus roseus cell suspension culture Cells, Cultured chemical constituents of plants chemical structure Chromatography, Liquid General pharmacology In Vitro Techniques Indicators and Reagents linoleic acid Linoleic Acid - isolation & purification Linoleic Acid - metabolism Linoleic Acid - pharmacology Medical sciences Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - chemistry Plant Extracts - pharmacology Purinergic P1 Receptor Antagonists Radioligand Assay Rats receptors Receptors, Opioid - metabolism Receptors, Purinergic P1 - metabolism Rubia tinctorum screening spectral analysis Tabernaemontana tabernaemontana pachysiphon Tabernaemontana pandacaqui |
| title | Interference of Linoleic Acid Fraction in Some Receptor Binding Assays |
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