Interference of Linoleic Acid Fraction in Some Receptor Binding Assays

An extract of a suspension culture of Tabernaemontana pandacaqui Poir. was fractionated by centrifugal partition chromatography. Aliquots were tested in an adenosine A1 receptor binding assay. This led to the isolation and identification of linoleic acid, which proved to be a noncompetitive inhibito...

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Veröffentlicht in:	Journal of natural products (Washington, D.C.) D.C.), 1999-06, Vol.62 (6), p.912-914
Hauptverfasser:	Ingkaninan, K, von Frijtag Drabbe Künzel, J. K, IJzerman, A. P, Verpoorte, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Aconitum aconitum xhenryi adenosine Animals binding sites Biological and medical sciences Brain - metabolism Catharanthus roseus cell suspension culture Cells, Cultured chemical constituents of plants chemical structure Chromatography, Liquid General pharmacology In Vitro Techniques Indicators and Reagents linoleic acid Linoleic Acid - isolation & purification Linoleic Acid - metabolism Linoleic Acid - pharmacology Medical sciences Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - chemistry Plant Extracts - pharmacology Purinergic P1 Receptor Antagonists Radioligand Assay Rats receptors Receptors, Opioid - metabolism Receptors, Purinergic P1 - metabolism Rubia tinctorum screening spectral analysis Tabernaemontana tabernaemontana pachysiphon Tabernaemontana pandacaqui
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Beschreibung
Zusammenfassung:	An extract of a suspension culture of Tabernaemontana pandacaqui Poir. was fractionated by centrifugal partition chromatography. Aliquots were tested in an adenosine A1 receptor binding assay. This led to the isolation and identification of linoleic acid, which proved to be a noncompetitive inhibitor. This “false positive”effect also extended to some other binding assays.
ISSN:	0163-3864 1520-6025
DOI:	10.1021/np9805490