6,7-Dimethyllumazine as a potential ligand for selective recognition of adenine opposite an abasic site in DNA duplexes

6,7-Dimethyllumazine as a potential ligand for selective recognition of adenine opposite an abasic site in DNA duplexes

6,7-Dimethyllumazine more selectively binds to adenine (A) base opposite the abasic site in DNA duplexes (5'-TCC AGX[combining low line] GCA AC-3'/3'-AGG TCN CGT TG-5', X = AP site (Spacer C3), N= A, T, C and G) than the other three nucleobases with a dissociation constant K(d) o...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2008-01 (48), p.6588-6590
Hauptverfasser: Ye, Zhiqiang, Rajendar, Burki, Qing, Dai, Nishizawa, Seiichi, Teramae, Norio
Format: Artikel
Sprache:eng
Schlagworte:
Adenine - chemistry
Base Sequence
DNA - chemistry
Ligands
Pteridines - chemistry
Spectrometry, Fluorescence
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Zusammenfassung:6,7-Dimethyllumazine more selectively binds to adenine (A) base opposite the abasic site in DNA duplexes (5'-TCC AGX[combining low line] GCA AC-3'/3'-AGG TCN CGT TG-5', X = AP site (Spacer C3), N= A, T, C and G) than the other three nucleobases with a dissociation constant K(d) of ca. 1.0 microM; substituted methyl groups enhance the binding affinity to A and the selectivity for A over T, compared to the parent molecule, lumazine.
ISSN:1359-7345
1364-548X
DOI:10.1039/b816876h