Blood Biochemical Characteristics, Cecal microbiota and Short-chain Fatty Acid Composition in Fistula Implanted rats

We raised an experimental rat implanted with a cecal fistula and investigated various characteristics of fistula-implanted rats. Male F344/N Slc rats at 14 weeks of age were divided into three groups, the fistula group (n=5) which consisted of fistula-implanted rats, the sham group (n=7) which consisted of sham-operated rats, and the control group (n=7) which were not subjected to any surgical procedure. Four weeks after the fistula implantion surgery, we compared the blood biochemical indices, the microflora composition and the short-chain fatty acids (SCFA) concentration in cecal contents of fistula-implanted rats with those of sham-operated and control rats. The blood albumin concentration of the fistula group was significantly lower than that of the sham group and the control group, and the hematocrit value of the fistula group was significantly lower than that of the control group, but there were no significant differences in the SCFA concentration and the microflora composition among these three groups. In conclusion, it was considered that the fistula-implanted rats are useful for taking cecal contents and determining the microflora composition and the metabolites concentration at any time, without disturbing the physiological functions of the intestinal tract.