A simple HPLC method for simultaneous determination of mycophenolic acid and mycophenolic acid glucuronide in plasma

A reversed-phase high-performance liquid chromatographic method for the simultaneous determination of mycophenolic acid and its metabolite, mycophenolic acid glucuronide, is presented herein. Sample purification is limited to protein precipitation with acetonitrile. The analytes were separated on a...

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Veröffentlicht in:	Therapeutic drug monitoring 1999-06, Vol.21 (3), p.322-324
Hauptverfasser:	SVENSSON, J.-O, BRATTSTRÖM, T, SÄWE, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Biological and medical sciences Chromatography, High Pressure Liquid General pharmacology Glucuronates - blood Glucuronides Humans Medical sciences Molecular Structure Mycophenolic Acid - analogs & derivatives Mycophenolic Acid - blood Pharmacology. Drug treatments Time Factors
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Beschreibung
Zusammenfassung:	A reversed-phase high-performance liquid chromatographic method for the simultaneous determination of mycophenolic acid and its metabolite, mycophenolic acid glucuronide, is presented herein. Sample purification is limited to protein precipitation with acetonitrile. The analytes were separated on a C18 column with a mobile phase containing 30% acetonitrile and a 40 mm phosphoric acid buffer at pH 2.1 and measured with UV-detection at 215 nm.
ISSN:	0163-4356 1536-3694
DOI:	10.1097/00007691-199906000-00012