Bacterial Surface Engineering Utilizing Glucosamine Phosphate Derivatives as Cell Wall Precursor Surrogates

New neighbors: N‐Levulinoyl glucosamine phosphate derivatives were added to a culture media of lactic acid bacteria as bacterial cell wall precursors, which should be integrated into the bacterial cell wall. The incorporation was estimated by flow cytometry after specific fluorescence labeling of the surface‐displayed ketone moiety. The derivatives with acetylated hydroxyl groups showed good incorporation due to increased hydrophobicity.