Increased expression of interleukin-4 during liver allograft rejection
Background/Aims: To investigate the respective roles of interleukin-2 (IL-2) and IL-4 during rejection, we evaluated the expression of IL-2, IL-2 receptor and IL-4 in human liver allografts. Methods: Immunohistochemistry and RT-PCR were performed in liver biopsies. To determine the effects of immuno...
Gespeichert in:
| Veröffentlicht in: | Journal of hepatology 1999-05, Vol.30 (5), p.935-943 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 943 |
|---|---|
| container_issue | 5 |
| container_start_page | 935 |
| container_title | Journal of hepatology |
| container_volume | 30 |
| creator | Conti, Filomena Calmus, Yvon Rouer, Evelyne Gaulard, Philippe Louvel, Albert Houssin, Didier Zafrani, Elie Serge |
| description | Background/Aims: To investigate the respective roles of interleukin-2 (IL-2) and IL-4 during rejection, we evaluated the expression of IL-2, IL-2 receptor and IL-4 in human liver allografts.
Methods: Immunohistochemistry and RT-PCR were performed in liver biopsies. To determine the effects of immunosuppression and cholestasis in IL-4 production,
in vitro experiments were also designed.
Results: IL-2 protein and its mRNA were absent in the liver, with minimal expression of IL-2 receptor, during rejection. In contrast, IL-4 protein and its mRNA were highly expressed during acute and chronic rejection, whereas this expression was absent in stable liver transplant recipients.
In vitro, cyclosporine potently inhibited IL-2 and IL-2 receptor expression of activated mononuclear blood cells, but poorly inhibited IL-4 expression. Chenodeoxycholic acid decreased IL-2 and IL-2 receptor expression, but increased IL-4 expression.
Conclusions: During liver allograft rejection, IL-2 pathway is down-regulated, while IL-4 expression is increased by cholestasis and poorly inhibited by cyclosporine. These data suggest that IL-4 is involved in the mechanisms of liver allograft rejection in patients treated with cyclosporine. |
| doi_str_mv | 10.1016/S0168-8278(99)80150-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69811051</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168827899801500</els_id><sourcerecordid>69811051</sourcerecordid><originalsourceid>FETCH-LOGICAL-c390t-17aa9c1d9971cd9a29753b799bd0ca503513f18d811809bd099b2a8dc6575bfa3</originalsourceid><addsrcrecordid>eNqFkE1LAzEQhoMoWqs_QdmDiB5WJ81mNzmJFKuFggf1HNJktqRud2uyW_Tfm36g3rwkYfK8M8NDyBmFGwo0v32Jh0jFoBBXUl4LoBxS2CM9mkN85BndJ70f5IgchzAHAAYyOyRHFFjOxYD1yGhcG486oE3wc-kxBNfUSVMmrm7RV9i9uzrNEtt5V8-Syq3QJ7qqmpnXZZt4nKNpY-KEHJS6Cni6u_vkbfTwOnxKJ8-P4-H9JDVMQpvSQmtpqJWyoMZKPZAFZ9NCyqkFozkwTllJhRWUClgX489AC2tyXvBpqVmfXG77Ln3z0WFo1cIFg1Wla2y6oHIZoxC79AnfgsY3IXgs1dK7hfZfioJaC1QbgWptR0mpNgIVxNz5bkA3XaD9k9oai8DFDtDB6Kr0ujYu_HJFkWWMR-xui2G0sXLoVTAOa4PW-ahM2cb9s8k3rRmMlg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69811051</pqid></control><display><type>article</type><title>Increased expression of interleukin-4 during liver allograft rejection</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Conti, Filomena ; Calmus, Yvon ; Rouer, Evelyne ; Gaulard, Philippe ; Louvel, Albert ; Houssin, Didier ; Zafrani, Elie Serge</creator><creatorcontrib>Conti, Filomena ; Calmus, Yvon ; Rouer, Evelyne ; Gaulard, Philippe ; Louvel, Albert ; Houssin, Didier ; Zafrani, Elie Serge</creatorcontrib><description>Background/Aims: To investigate the respective roles of interleukin-2 (IL-2) and IL-4 during rejection, we evaluated the expression of IL-2, IL-2 receptor and IL-4 in human liver allografts.
Methods: Immunohistochemistry and RT-PCR were performed in liver biopsies. To determine the effects of immunosuppression and cholestasis in IL-4 production,
in vitro experiments were also designed.
Results: IL-2 protein and its mRNA were absent in the liver, with minimal expression of IL-2 receptor, during rejection. In contrast, IL-4 protein and its mRNA were highly expressed during acute and chronic rejection, whereas this expression was absent in stable liver transplant recipients.
In vitro, cyclosporine potently inhibited IL-2 and IL-2 receptor expression of activated mononuclear blood cells, but poorly inhibited IL-4 expression. Chenodeoxycholic acid decreased IL-2 and IL-2 receptor expression, but increased IL-4 expression.
Conclusions: During liver allograft rejection, IL-2 pathway is down-regulated, while IL-4 expression is increased by cholestasis and poorly inhibited by cyclosporine. These data suggest that IL-4 is involved in the mechanisms of liver allograft rejection in patients treated with cyclosporine.</description><identifier>ISSN: 0168-8278</identifier><identifier>EISSN: 1600-0641</identifier><identifier>DOI: 10.1016/S0168-8278(99)80150-0</identifier><identifier>PMID: 10365823</identifier><identifier>CODEN: JOHEEC</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Acute Disease ; Adult ; Allograft rejection ; Biological and medical sciences ; Cholestasis ; Chronic Disease ; Cyclosporine - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - immunology ; Graft Rejection - immunology ; Graft Rejection - pathology ; Humans ; Immunohistochemistry ; Immunosuppression ; Interleukin-2 - analysis ; Interleukin-2 - genetics ; Interleukin-4 ; Interleukin-4 - analysis ; Interleukin-4 - genetics ; Liver transplantation ; Liver Transplantation - immunology ; Liver Transplantation - pathology ; Male ; Middle Aged ; Postoperative Complications - immunology ; Postoperative Complications - pathology ; Receptors, Interleukin-2 - analysis ; Receptors, Interleukin-2 - genetics ; Retrospective Studies ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Tissue, organ and graft immunology ; Transcription, Genetic ; Transplantation, Homologous</subject><ispartof>Journal of hepatology, 1999-05, Vol.30 (5), p.935-943</ispartof><rights>1999</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-17aa9c1d9971cd9a29753b799bd0ca503513f18d811809bd099b2a8dc6575bfa3</citedby><cites>FETCH-LOGICAL-c390t-17aa9c1d9971cd9a29753b799bd0ca503513f18d811809bd099b2a8dc6575bfa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0168-8278(99)80150-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1774435$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10365823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Conti, Filomena</creatorcontrib><creatorcontrib>Calmus, Yvon</creatorcontrib><creatorcontrib>Rouer, Evelyne</creatorcontrib><creatorcontrib>Gaulard, Philippe</creatorcontrib><creatorcontrib>Louvel, Albert</creatorcontrib><creatorcontrib>Houssin, Didier</creatorcontrib><creatorcontrib>Zafrani, Elie Serge</creatorcontrib><title>Increased expression of interleukin-4 during liver allograft rejection</title><title>Journal of hepatology</title><addtitle>J Hepatol</addtitle><description>Background/Aims: To investigate the respective roles of interleukin-2 (IL-2) and IL-4 during rejection, we evaluated the expression of IL-2, IL-2 receptor and IL-4 in human liver allografts.
Methods: Immunohistochemistry and RT-PCR were performed in liver biopsies. To determine the effects of immunosuppression and cholestasis in IL-4 production,
in vitro experiments were also designed.
Results: IL-2 protein and its mRNA were absent in the liver, with minimal expression of IL-2 receptor, during rejection. In contrast, IL-4 protein and its mRNA were highly expressed during acute and chronic rejection, whereas this expression was absent in stable liver transplant recipients.
In vitro, cyclosporine potently inhibited IL-2 and IL-2 receptor expression of activated mononuclear blood cells, but poorly inhibited IL-4 expression. Chenodeoxycholic acid decreased IL-2 and IL-2 receptor expression, but increased IL-4 expression.
Conclusions: During liver allograft rejection, IL-2 pathway is down-regulated, while IL-4 expression is increased by cholestasis and poorly inhibited by cyclosporine. These data suggest that IL-4 is involved in the mechanisms of liver allograft rejection in patients treated with cyclosporine.</description><subject>Acute Disease</subject><subject>Adult</subject><subject>Allograft rejection</subject><subject>Biological and medical sciences</subject><subject>Cholestasis</subject><subject>Chronic Disease</subject><subject>Cyclosporine - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - immunology</subject><subject>Graft Rejection - immunology</subject><subject>Graft Rejection - pathology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunosuppression</subject><subject>Interleukin-2 - analysis</subject><subject>Interleukin-2 - genetics</subject><subject>Interleukin-4</subject><subject>Interleukin-4 - analysis</subject><subject>Interleukin-4 - genetics</subject><subject>Liver transplantation</subject><subject>Liver Transplantation - immunology</subject><subject>Liver Transplantation - pathology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Postoperative Complications - immunology</subject><subject>Postoperative Complications - pathology</subject><subject>Receptors, Interleukin-2 - analysis</subject><subject>Receptors, Interleukin-2 - genetics</subject><subject>Retrospective Studies</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Tissue, organ and graft immunology</subject><subject>Transcription, Genetic</subject><subject>Transplantation, Homologous</subject><issn>0168-8278</issn><issn>1600-0641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMoWqs_QdmDiB5WJ81mNzmJFKuFggf1HNJktqRud2uyW_Tfm36g3rwkYfK8M8NDyBmFGwo0v32Jh0jFoBBXUl4LoBxS2CM9mkN85BndJ70f5IgchzAHAAYyOyRHFFjOxYD1yGhcG486oE3wc-kxBNfUSVMmrm7RV9i9uzrNEtt5V8-Syq3QJ7qqmpnXZZt4nKNpY-KEHJS6Cni6u_vkbfTwOnxKJ8-P4-H9JDVMQpvSQmtpqJWyoMZKPZAFZ9NCyqkFozkwTllJhRWUClgX489AC2tyXvBpqVmfXG77Ln3z0WFo1cIFg1Wla2y6oHIZoxC79AnfgsY3IXgs1dK7hfZfioJaC1QbgWptR0mpNgIVxNz5bkA3XaD9k9oai8DFDtDB6Kr0ujYu_HJFkWWMR-xui2G0sXLoVTAOa4PW-ahM2cb9s8k3rRmMlg</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>Conti, Filomena</creator><creator>Calmus, Yvon</creator><creator>Rouer, Evelyne</creator><creator>Gaulard, Philippe</creator><creator>Louvel, Albert</creator><creator>Houssin, Didier</creator><creator>Zafrani, Elie Serge</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990501</creationdate><title>Increased expression of interleukin-4 during liver allograft rejection</title><author>Conti, Filomena ; Calmus, Yvon ; Rouer, Evelyne ; Gaulard, Philippe ; Louvel, Albert ; Houssin, Didier ; Zafrani, Elie Serge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-17aa9c1d9971cd9a29753b799bd0ca503513f18d811809bd099b2a8dc6575bfa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acute Disease</topic><topic>Adult</topic><topic>Allograft rejection</topic><topic>Biological and medical sciences</topic><topic>Cholestasis</topic><topic>Chronic Disease</topic><topic>Cyclosporine - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene Expression Regulation - immunology</topic><topic>Graft Rejection - immunology</topic><topic>Graft Rejection - pathology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Immunosuppression</topic><topic>Interleukin-2 - analysis</topic><topic>Interleukin-2 - genetics</topic><topic>Interleukin-4</topic><topic>Interleukin-4 - analysis</topic><topic>Interleukin-4 - genetics</topic><topic>Liver transplantation</topic><topic>Liver Transplantation - immunology</topic><topic>Liver Transplantation - pathology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Postoperative Complications - immunology</topic><topic>Postoperative Complications - pathology</topic><topic>Receptors, Interleukin-2 - analysis</topic><topic>Receptors, Interleukin-2 - genetics</topic><topic>Retrospective Studies</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Tissue, organ and graft immunology</topic><topic>Transcription, Genetic</topic><topic>Transplantation, Homologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Conti, Filomena</creatorcontrib><creatorcontrib>Calmus, Yvon</creatorcontrib><creatorcontrib>Rouer, Evelyne</creatorcontrib><creatorcontrib>Gaulard, Philippe</creatorcontrib><creatorcontrib>Louvel, Albert</creatorcontrib><creatorcontrib>Houssin, Didier</creatorcontrib><creatorcontrib>Zafrani, Elie Serge</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Conti, Filomena</au><au>Calmus, Yvon</au><au>Rouer, Evelyne</au><au>Gaulard, Philippe</au><au>Louvel, Albert</au><au>Houssin, Didier</au><au>Zafrani, Elie Serge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased expression of interleukin-4 during liver allograft rejection</atitle><jtitle>Journal of hepatology</jtitle><addtitle>J Hepatol</addtitle><date>1999-05-01</date><risdate>1999</risdate><volume>30</volume><issue>5</issue><spage>935</spage><epage>943</epage><pages>935-943</pages><issn>0168-8278</issn><eissn>1600-0641</eissn><coden>JOHEEC</coden><abstract>Background/Aims: To investigate the respective roles of interleukin-2 (IL-2) and IL-4 during rejection, we evaluated the expression of IL-2, IL-2 receptor and IL-4 in human liver allografts.
Methods: Immunohistochemistry and RT-PCR were performed in liver biopsies. To determine the effects of immunosuppression and cholestasis in IL-4 production,
in vitro experiments were also designed.
Results: IL-2 protein and its mRNA were absent in the liver, with minimal expression of IL-2 receptor, during rejection. In contrast, IL-4 protein and its mRNA were highly expressed during acute and chronic rejection, whereas this expression was absent in stable liver transplant recipients.
In vitro, cyclosporine potently inhibited IL-2 and IL-2 receptor expression of activated mononuclear blood cells, but poorly inhibited IL-4 expression. Chenodeoxycholic acid decreased IL-2 and IL-2 receptor expression, but increased IL-4 expression.
Conclusions: During liver allograft rejection, IL-2 pathway is down-regulated, while IL-4 expression is increased by cholestasis and poorly inhibited by cyclosporine. These data suggest that IL-4 is involved in the mechanisms of liver allograft rejection in patients treated with cyclosporine.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>10365823</pmid><doi>10.1016/S0168-8278(99)80150-0</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-8278 |
| ispartof | Journal of hepatology, 1999-05, Vol.30 (5), p.935-943 |
| issn | 0168-8278 1600-0641 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69811051 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acute Disease Adult Allograft rejection Biological and medical sciences Cholestasis Chronic Disease Cyclosporine - pharmacology Female Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Expression Regulation - drug effects Gene Expression Regulation - immunology Graft Rejection - immunology Graft Rejection - pathology Humans Immunohistochemistry Immunosuppression Interleukin-2 - analysis Interleukin-2 - genetics Interleukin-4 Interleukin-4 - analysis Interleukin-4 - genetics Liver transplantation Liver Transplantation - immunology Liver Transplantation - pathology Male Middle Aged Postoperative Complications - immunology Postoperative Complications - pathology Receptors, Interleukin-2 - analysis Receptors, Interleukin-2 - genetics Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Tissue, organ and graft immunology Transcription, Genetic Transplantation, Homologous |
| title | Increased expression of interleukin-4 during liver allograft rejection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T21%3A37%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20expression%20of%20interleukin-4%20during%20liver%20allograft%20rejection&rft.jtitle=Journal%20of%20hepatology&rft.au=Conti,%20Filomena&rft.date=1999-05-01&rft.volume=30&rft.issue=5&rft.spage=935&rft.epage=943&rft.pages=935-943&rft.issn=0168-8278&rft.eissn=1600-0641&rft.coden=JOHEEC&rft_id=info:doi/10.1016/S0168-8278(99)80150-0&rft_dat=%3Cproquest_cross%3E69811051%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69811051&rft_id=info:pmid/10365823&rft_els_id=S0168827899801500&rfr_iscdi=true |