Increased expression of interleukin-4 during liver allograft rejection
Background/Aims: To investigate the respective roles of interleukin-2 (IL-2) and IL-4 during rejection, we evaluated the expression of IL-2, IL-2 receptor and IL-4 in human liver allografts. Methods: Immunohistochemistry and RT-PCR were performed in liver biopsies. To determine the effects of immuno...
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Veröffentlicht in: | Journal of hepatology 1999-05, Vol.30 (5), p.935-943 |
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Sprache: | eng |
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Zusammenfassung: | Background/Aims: To investigate the respective roles of interleukin-2 (IL-2) and IL-4 during rejection, we evaluated the expression of IL-2, IL-2 receptor and IL-4 in human liver allografts.
Methods: Immunohistochemistry and RT-PCR were performed in liver biopsies. To determine the effects of immunosuppression and cholestasis in IL-4 production,
in vitro experiments were also designed.
Results: IL-2 protein and its mRNA were absent in the liver, with minimal expression of IL-2 receptor, during rejection. In contrast, IL-4 protein and its mRNA were highly expressed during acute and chronic rejection, whereas this expression was absent in stable liver transplant recipients.
In vitro, cyclosporine potently inhibited IL-2 and IL-2 receptor expression of activated mononuclear blood cells, but poorly inhibited IL-4 expression. Chenodeoxycholic acid decreased IL-2 and IL-2 receptor expression, but increased IL-4 expression.
Conclusions: During liver allograft rejection, IL-2 pathway is down-regulated, while IL-4 expression is increased by cholestasis and poorly inhibited by cyclosporine. These data suggest that IL-4 is involved in the mechanisms of liver allograft rejection in patients treated with cyclosporine. |
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ISSN: | 0168-8278 1600-0641 |
DOI: | 10.1016/S0168-8278(99)80150-0 |