Mapping of the Antigenic Regions of Streptokinase in Humans after Streptokinase Therapy

Streptokinase (SK) is efficaciously used as a thrombolytic drug for the treatment of myocardial infarction. Being a bacterial protein, SK is immunogenic in humans. Therefore, resulting from SK therapy, patients become immunized and anti-SK antibody (Ab) titers rise post-treatment. High Ab titers mig...

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Veröffentlicht in:Biochemical and biophysical research communications 1999-05, Vol.259 (1), p.162-168
Hauptverfasser: Torréns, Isis, Reyes, Osvaldo, Ojalvo, Ariana G., Seralena, Alina, Chinea, Glay, Cruz, Luis J., de la Fuente, José
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Sprache:eng
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Zusammenfassung:Streptokinase (SK) is efficaciously used as a thrombolytic drug for the treatment of myocardial infarction. Being a bacterial protein, SK is immunogenic in humans. Therefore, resulting from SK therapy, patients become immunized and anti-SK antibody (Ab) titers rise post-treatment. High Ab titers might provoke severe immune reactions during SK therapy and neutralize SK activity, preventing effective thrombolysis. Spot synthesis combined with peptide library techniques is a useful tool for studying protein-peptide interactions on continuous cellulose membranes. Here, we report on the mapping of antigenic regions of SK using a spot-synthesized peptide library and human total sera from patients receiving SK therapy. All tested samples have high anti-SK Ab titers and most of them show significant SK neutralizing capacity. Individual variations in peptide recognition were detected. However, patients treated with SK tend, in general, to show a common regional binding pattern, including residues 1-20, 130-149, 170-189, and 390-399. This is the first study reporting the probing of a cellulose-bound set of peptides with total human sera.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.0747