A latex agglutination assay for specific detection of Panton–Valentine leukocidin

Panton–Valentine leukocidin (PVL) is produced by some isolates of Staphylococcus aureus, and has been associated with the high pathogenicpotential of these strains. To rapidly detect the toxin producer strains, we developed a reverse passive latex agglutination (RPLA) reaction assay specific for PVL...

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Veröffentlicht in:	Journal of microbiological methods 2008-12, Vol.75 (3), p.411-415
Hauptverfasser:	Oishi, Kanenari, Baba, Tadashi, Nakatomi, Yasuo, Ito, Teruyo, Hiramatsu, Keiichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Toxins - genetics Bacterial Toxins - metabolism Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Exotoxins - genetics Exotoxins - metabolism Fundamental and applied biological sciences. Psychology Humans Latex Fixation Tests - methods Leukocidins - genetics Leukocidins - metabolism Microbiology Panton–Valentine leukocidin Rabbits Reverse passive latex agglutination Sensitivity and Specificity Staphylococcal Infections - diagnosis Staphylococcal Infections - microbiology Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Staphylococcus aureus - metabolism Toxin producer strain
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Beschreibung
Zusammenfassung:	Panton–Valentine leukocidin (PVL) is produced by some isolates of Staphylococcus aureus, and has been associated with the high pathogenicpotential of these strains. To rapidly detect the toxin producer strains, we developed a reverse passive latex agglutination (RPLA) reaction assay specific for PVL. By testing 64 S. aureus strains, the assay could detect the 35 pvl-gene-positive strains with 100% specificity and sensitivity. Furthermore, the assay revealed an extensive variation in the amount of PVL produced by the pvl-positive strains.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2008.07.013