Polysaccharides from Dioscorea batatas Induce Tumor Necrosis Factor-α Secretion via Toll-like Receptor 4-Mediated Protein Kinase Signaling Pathways

The bioactive polysaccharides (named ZPF1) from yam (Dioscorea batatas) were chemically determined, suggesting repeating β-1,4-mannan as mainly having a feature of acetylation on C2−OH and C3−OH, around 28%. The ZPF1 participated in the stimulation of murine wild-type macrophages predominantly in tu...

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Veröffentlicht in:Journal of agricultural and food chemistry 2008-11, Vol.56 (21), p.9892-9898
Hauptverfasser: Liu, Ju-Yun, Yang, Feng-Ling, Lu, Chun-Ping, Yang, Yu-Liang, Wen, Chi-Luan, Hua, Kuo-Feng, Wu, Shih-Hsiung
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Sprache:eng
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Zusammenfassung:The bioactive polysaccharides (named ZPF1) from yam (Dioscorea batatas) were chemically determined, suggesting repeating β-1,4-mannan as mainly having a feature of acetylation on C2−OH and C3−OH, around 28%. The ZPF1 participated in the stimulation of murine wild-type macrophages predominantly in tumor necrosis factor-α (TNFα). Toll-like receptor 4 is proved to be one of the cellular receptors in ZPF1-mediated TNFα secretion. Reactive oxygen species transmission and PI3-kinase are found necessary for regulating TNFα secretion by ZPF1 stimulation. Moreover, we found that extracellular signal-regulated kinase 1/2, Jun N-terminal kinase 1/2, and p38 mitogen-activated protein kinase play important roles in the regulation of TNFα secretion in ZPF1-stimulated macrophages.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf8018722