Two supporting factors greatly improve the efficiency of human iPSC generation

Human fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4, and c- MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We fo...

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Veröffentlicht in:	Cell stem cell 2008-11, Vol.3 (5), p.475-479
Hauptverfasser:	Zhao, Yang, Yin, Xiaolei, Qin, Han, Zhu, Fangfang, Liu, Haisong, Yang, Weifeng, Zhang, Qiang, Xiang, Chengang, Hou, Pingping, Song, Zhihua, Liu, Yanxia, Yong, Jun, Zhang, Pengbo, Cai, Jun, Liu, Meng, Li, Honggang, Li, Yanqin, Qu, Xiuxia, Cui, Kai, Zhang, Weiqi, Xiang, Tingting, Wu, Yetao, Zhao, Yiding, Liu, Chun, Yu, Chen, Yuan, Kehu, Lou, Jinning, Ding, Mingxiao, Deng, Hongkui
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Cell Culture Techniques Cell Differentiation - genetics Cellular Reprogramming - genetics Colony-Forming Units Assay Fibroblasts - cytology Fibroblasts - metabolism Foreskin - cytology Gene Regulatory Networks - genetics Humans Male Nuclear Proteins - genetics Nuclear Proteins - metabolism Pluripotent Stem Cells - cytology Pluripotent Stem Cells - physiology RNA, Small Interfering Trans-Activators - genetics Trans-Activators - metabolism Transfection Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - metabolism
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Beschreibung
Zusammenfassung:	Human fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4, and c- MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPSC generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations.
ISSN:	1934-5909 1875-9777
DOI:	10.1016/j.stem.2008.10.002