Microbiological and histological study of the gastrointestinal tract of germ-free mice infected with Helicobacter trogontum

Helicobacter spp. have been the focus of considerable research because of the role of this genus in gastrointestinal diseases. We infected NIH germ-free mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the distribution of this bacterium in the ga...

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Veröffentlicht in:Research in microbiology 1999-04, Vol.150 (3), p.205-212
Hauptverfasser: Moura, Silvia B, Mendes, Edilberto N, Queiroz, Dulciene M. M, Nicoli, Jacques R, Cabral, Mônica M. D. A, Magalhaes, Paula P, Rocha, Gifone A, Vieira, Ênio C
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Sprache:eng
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Zusammenfassung:Helicobacter spp. have been the focus of considerable research because of the role of this genus in gastrointestinal diseases. We infected NIH germ-free mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the distribution of this bacterium in the gastrointestinal tract and the histopathological changes it can induce in this host. Sixteen mice were challenged with a single dose of H. trogontum (test group) and killed one and six weeks after inoculation (eight animals at each point). Eight animals were challenged with 0.85% saline alone (control group) and killed at the same time points (four at each point). Fragments from the gastric and intestinal mucosa were obtained for microbiological and histological examination. H. trogontum was isolated from the cecum and colon of all test mice and also from the gastric mucosa of several of them. All infected animals presented histological changes in at least one region of the bowel. Alterations in the gastric mucosa were also observed mainly in the six-week-infected group. The predominant histological change observed was a moderate diffuse inflammatory infiltrate of mononuclear cells in the lamina propria, often accompanied by a mild infiltration of polymorphonuclear cells. Two animals presented focal infiltration of inflammatory cells in the liver, although no bacteria were found in the liver of any animal. H. trogontum is an intestinal species that is able to elicit inflammatory responses in other regions of the gastrointestinal tract such as the gastric mucosa and the liver of gnotobioticmice. Études microbiologique et histologique du tractus gastrointestinal de souris axéniques infectées par Helicobacter trogontum . Le genre Helicobacter a été l'objet de nombreuses recherches en fonction de son rôle dans certaines maladies gastrointestinales. Nous avons infecté des souris axéniques avec H. trogontum, une bactérie intestinale du rat récemment décrite, afin d'étudier la distribution de ce microorganisme dans le tractus gastrointestinal et les modifications histopathologiques qu'il induit chez l'hôte. Seize souris ont été inoculées avec une dose unique de H. trogontum (groupe test) et sacrifiées une et six semaines après l'inoculation (huit animaux chaque fois). Huit animaux ont été inoculés avec une solution saline à 0,85 % (groupe témoin) et sacrifiés aux mêmes temps (quatre chaque fois). Des fragments des muqueuses gastrique et intestinale ont été soumis à des e
ISSN:0923-2508
1769-7123
DOI:10.1016/S0923-2508(99)80037-0