Simultaneous Measurement of Serum Testosterone and Dihydrotestosterone by Liquid Chromatography-Tandem Mass Spectrometry

Simultaneous Measurement of Serum Testosterone and Dihydrotestosterone by Liquid Chromatography-Tandem Mass Spectrometry

Recent reports have described inherent problems with androgen immunoassays compared with mass spectrometry analyses. We developed a method for measuring serum testosterone (T) and 5alpha-dihydrotestosterone (DHT) simultaneously via liquid-liquid extraction followed by liquid chromatography-tandem ma...

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Veröffentlicht in:Clinical chemistry (Baltimore, Md.) Md.), 2008-11, Vol.54 (11), p.1855-1863
Hauptverfasser: Shiraishi, Steve, Lee, Paul W. N, Leung, Andrew, Goh, Victor H. H, Swerdloff, Ronald S, Wang, Christina
Format: Artikel
Sprache:eng
Schlagworte:
Adult
Analytical, structural and metabolic biochemistry
Androgens
Aqueous solutions
Biological and medical sciences
Biomedical research
Calibration
Chromatography, Liquid - methods
Dihydrotestosterone - blood
Female
Females
Fundamental and applied biological sciences. Psychology
Humans
Immunoassays
Investigative techniques, diagnostic techniques (general aspects)
Ionization
Liquid chromatography
Male
Mass spectrometry
Medical sciences
Methods
Middle Aged
Physical examinations
Radioimmunoassay
Reference Values
Research centers
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry - methods
Testosterone
Testosterone - blood
Validation studies
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Zusammenfassung:Recent reports have described inherent problems with androgen immunoassays compared with mass spectrometry analyses. We developed a method for measuring serum testosterone (T) and 5alpha-dihydrotestosterone (DHT) simultaneously via liquid-liquid extraction followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with positive-mode electrospray ionization. The DHT and T calibrators showed a linear response from 0.069 nmol/L to 34.4 nmol/L and 69.3 nmol/L, respectively. T interference in the DHT assay and vice versa were negligible. Within- and between-run imprecision values were
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2008.103846