Crystallization and preliminary X-ray crystallographic studies of RepDC, a hybrid rolling-circle plasmid replication initiator protein
The hybrid plasmid‐replication initiator protein RepDC, which is a fusion of the catalytic fragment of the RepD protein and the DNA‐binding fragment of the RepC protein from Staphylococcus aureus, has been successfully crystallized and X‐ray data to 3.5 Å have been collected on a synchrotron radiati...
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| Veröffentlicht in: | Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 1999-05, Vol.55 (5), p.1076-1078 |
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| container_end_page | 1078 |
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| container_issue | 5 |
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| container_title | Acta crystallographica. Section D, Biological crystallography. |
| container_volume | 55 |
| creator | Klimenko, Dmitry E. Convery, Máire A. Rowsell, Siân Thomas, Christopher D. Phillips, Simon E. V. |
| description | The hybrid plasmid‐replication initiator protein RepDC, which is a fusion of the catalytic fragment of the RepD protein and the DNA‐binding fragment of the RepC protein from Staphylococcus aureus, has been successfully crystallized and X‐ray data to 3.5 Å have been collected on a synchrotron radiation source. Crystals belong to space group I4132 with unit‐cell dimensions a = b = c = 165.1 Å. The crystals are estimated to contain one protein monomer per asymmetric unit, with 55% solvent content. |
| doi_str_mv | 10.1107/S0907444999003005 |
| format | Article |
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The crystals are estimated to contain one protein monomer per asymmetric unit, with 55% solvent content.</description><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Crystallization</subject><subject>Crystallography, X-Ray</subject><subject>DNA Helicases - chemistry</subject><subject>DNA Helicases - genetics</subject><subject>DNA, Circular - chemistry</subject><subject>DNA, Circular - genetics</subject><subject>DNA-Binding Proteins</subject><subject>plasmid-replication initiation proteins</subject><subject>Plasmids - chemistry</subject><subject>Protein Conformation</subject><subject>RepDC</subject><subject>Staphylococcus aureus - chemistry</subject><subject>Trans-Activators - chemistry</subject><subject>Trans-Activators - genetics</subject><issn>1399-0047</issn><issn>0907-4449</issn><issn>1399-0047</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkN1qFTEUhYNYbK0-gDeSK68cu_Mzk-TyMMe2QqtwVKxXISeTaaOZnyZzsOMD-NxNmYMUvBA2ZO-wvrVgIfSKwDtCQJx8BgWCc66UAmAA5RN0RJhSBQAXTx_th-h5Sj8AgFImnqFDApRUjJAj9KeOc5pMCP63mfzQY9M3eIwu-M73Js74qohmxnavGq6jGW-8xWnaNd4lPLR448Z1_RYbfDNvo29wHLJbf11YH21weAwmdQ_fbgzeLiG-95M30xBz1DA5379AB60Jyb3cv8fo6-n7L_V5cfHp7EO9uigsk5wXlMlGCiqdozRPC7JtHUjghJeNgcqyfJZiS0ARSwyHlhpJSy6Ya1uqFDtGbxbfnHu7c2nSnU_WhWB6N-ySrpQgXDKahWQR2jikFF2rx-i7XIgmoB_K1_-Un5nXe_PdtnPNI2JpOwvkIvjlg5v_76hX39erNUDFM1osqE-Tu_uLmvhTV4KJUn_7eKary6vN-pTVesPuAe1XoIw</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>Klimenko, Dmitry E.</creator><creator>Convery, Máire A.</creator><creator>Rowsell, Siân</creator><creator>Thomas, Christopher D.</creator><creator>Phillips, Simon E. 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D</addtitle><date>1999-05-01</date><risdate>1999</risdate><volume>55</volume><issue>5</issue><spage>1076</spage><epage>1078</epage><pages>1076-1078</pages><issn>1399-0047</issn><issn>0907-4449</issn><eissn>1399-0047</eissn><abstract>The hybrid plasmid‐replication initiator protein RepDC, which is a fusion of the catalytic fragment of the RepD protein and the DNA‐binding fragment of the RepC protein from Staphylococcus aureus, has been successfully crystallized and X‐ray data to 3.5 Å have been collected on a synchrotron radiation source. Crystals belong to space group I4132 with unit‐cell dimensions a = b = c = 165.1 Å. The crystals are estimated to contain one protein monomer per asymmetric unit, with 55% solvent content.</abstract><cop>5 Abbey Square, Chester, Cheshire CH1 2HU, England</cop><pub>International Union of Crystallography</pub><pmid>10216311</pmid><doi>10.1107/S0907444999003005</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 1399-0047 |
| ispartof | Acta crystallographica. Section D, Biological crystallography., 1999-05, Vol.55 (5), p.1076-1078 |
| issn | 1399-0047 0907-4449 1399-0047 |
| language | eng |
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| source | MEDLINE; Wiley Journals; Crystallography Journals Online; Alma/SFX Local Collection |
| subjects | Bacterial Proteins - chemistry Bacterial Proteins - genetics Crystallization Crystallography, X-Ray DNA Helicases - chemistry DNA Helicases - genetics DNA, Circular - chemistry DNA, Circular - genetics DNA-Binding Proteins plasmid-replication initiation proteins Plasmids - chemistry Protein Conformation RepDC Staphylococcus aureus - chemistry Trans-Activators - chemistry Trans-Activators - genetics |
| title | Crystallization and preliminary X-ray crystallographic studies of RepDC, a hybrid rolling-circle plasmid replication initiator protein |
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