Crystallization and preliminary X-ray crystallographic studies of RepDC, a hybrid rolling-circle plasmid replication initiator protein

The hybrid plasmid‐replication initiator protein RepDC, which is a fusion of the catalytic fragment of the RepD protein and the DNA‐binding fragment of the RepC protein from Staphylococcus aureus, has been successfully crystallized and X‐ray data to 3.5 Å have been collected on a synchrotron radiation source. Crystals belong to space group I4132 with unit‐cell dimensions a = b = c = 165.1 Å. The crystals are estimated to contain one protein monomer per asymmetric unit, with 55% solvent content.