Preparation of IgG Subclass Allotypes from Polyclonal IgG

Two allotypes have been identified for each of the IgG subclasses IgG1, IgG2 and IgG3. These allotypes are referred to as G1m(a) and G1m(f), G2m(n) and G2m(‐n), and G3m(g) and G3m(b). Using a pool of normal human serum and a combination of preparative electrophoresis, DEAE ion‐exchange and protein A...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Scandinavian journal of immunology 1999-04, Vol.49 (4), p.395-398
1. Verfasser:	Oxelius, V A
Format:	Artikel
Sprache:	eng
Schlagworte:	Blood Protein Electrophoresis Chromatography, Affinity Chromatography, Ion Exchange Humans Immunoglobulin Allotypes - blood Immunoglobulin Allotypes - isolation & purification Immunoglobulin G - blood Immunoglobulin G - isolation & purification Immunoglobulin Gm Allotypes - blood Sepharose - analogs & derivatives
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Two allotypes have been identified for each of the IgG subclasses IgG1, IgG2 and IgG3. These allotypes are referred to as G1m(a) and G1m(f), G2m(n) and G2m(‐n), and G3m(g) and G3m(b). Using a pool of normal human serum and a combination of preparative electrophoresis, DEAE ion‐exchange and protein A–Sepharose chromatography, it was possible to separate G1m(f) from G1m(a), G2m(‐n) from G2m(n) and G3m(g) from G3m(b). Purification of G2m(‐n) molecules is of special interest as no genetic marker has been found to identify this allotype.
ISSN:	0300-9475 1365-3083
DOI:	10.1046/j.1365-3083.1999.00521.x