A novel inhibitor protein for Bombyx cysteine proteinase is homologous to propeptide regions of cysteine proteinases

A cDNA clone for an inhibitor of Bombyx cysteine proteinase was isolated and sequenced. Active inhibitor proteins were expressed in Escherichia coli using the cDNA. The open reading frame of the cDNA encodes a 105 residues protein with 19 residues of a signal sequence. The inhibitor has amino acid s...

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Veröffentlicht in:	FEBS letters 1999-04, Vol.448 (2), p.257-260
Hauptverfasser:	Yamamoto, Yoshimi, Watabe, Shoji, Kageyama, Takashi, Takahashi, Susumu Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Blotting, Northern Bombyx - enzymology cDNA cloning Cloning, Molecular Cysteine Endopeptidases - chemistry Cysteine proteinase Cysteine Proteinase Inhibitors - genetics Databases, Factual Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Inhibitor Models, Genetic Molecular Sequence Data Propeptide region Sequence homology Sequence Homology, Amino Acid
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Zusammenfassung:	A cDNA clone for an inhibitor of Bombyx cysteine proteinase was isolated and sequenced. Active inhibitor proteins were expressed in Escherichia coli using the cDNA. The open reading frame of the cDNA encodes a 105 residues protein with 19 residues of a signal sequence. The inhibitor has amino acid sequences homologous to several cysteine proteinases, but only to their propeptide sequences. The results suggest that some cysteine proteinase proregions may have evolved as autonomous modules and become inhibitor proteins for cysteine proteinases.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/S0014-5793(99)00382-8